Histone Methyltransferases - Methods and Protocols (Hardcover, 1st ed. 2022)

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

General

Imprint:	Springer-Verlag New York
Country of origin:	United States
Series:	Methods in Molecular Biology, 2529
Release date:	July 2022
First published:	2022
Editors:	Raphael Margueron • Daniel Holoch
Dimensions:	254 x 178mm (L x W)
Format:	Hardcover
Pages:	495
Edition:	1st ed. 2022
ISBN-13:	978-1-07-162480-7
Categories:	Books > Science & Mathematics > Biology, life sciences > Biochemistry > Enzymology Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > General
LSN:	1-07-162480-6
Barcode:	9781071624807

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