Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Protein Synthesis - Methods and Protocols (Hardcover, 1998 ed.)
Loot Price: R4,625
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Protein Synthesis - Methods and Protocols (Hardcover, 1998 ed.)
Series: Methods in Molecular Biology, 77
Expected to ship within 10 - 15 working days
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The synthesis of proteins from 20 or so constituent amino acids
according to a strictly defined code with an accuracy of better
than 1 in 10,000 at most loca tions is arguably the most complex
task performed by cells. Protein Synthesis collects together
methods and protocols covering a range of different approaches
towards understanding how the cellular machinery accomplishes this
task and how these ftinctions might be harnessed by the
biotechnology industry to generate novel and useful proteins. The
era in which the components of the translational machinery were
being catalogued is over. This volume gathers together protocols
that focus on preserving and describing the dynamic function as
closely as possible. The need to understand exactly how ribosomes
are positioned on messages or where tRNA molecules, translation
factors, or control proteins are bound, has been appreciated by
many of the authors. Several chapters that explore the fidelity and
processivity of translation reflect this belief. Moreover, the
fundamental importance of rRNA at the heart of the ribosome is a
strong theme in a number of the protocols. These articles include
in vitro and in vivo systems from bacterial, fungal, plant, and
animal systems. Overall, Protein Synthesis might be characterized
by the novelty of the approaches employed to illuminate the inner
workings of the protein synthetic machinery as well as by the
inventiveness of the attempts to harness these reactions for
biotechnological applications."
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