Immunohistochemistry and immunocytochemistry are invaluable tools
for the visualization of tissue and cellular antigens in diagnostic
and biological research environments. The need to obtain accurate,
reliable and reproducible results is paramount. It is with this
fundamental aim in mind that we have compiled Immunohistochemistry:
Essential Methods. We have achieved this by examining each aspect
of immunochemistry in turn, with each chapter including detailed
information regarding the subject matter in question. Each chapter
is written by an expert in their field and includes protocols that
are typically used in their own research. Subjects covered are,
amongst others, antibodies and their production; selection of
reporter labels; immunochemical staining methods and experimental
design (both using single and multiple reporter labels); quality
assurance; automated immunochemistry; confocal microscopy and
electron microscopy. In addition, benefits and limitations of each
approach are discussed within the chapters.
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