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Phytochemical Investigation of Citrus limetta Peel Oil (Paperback)
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Phytochemical Investigation of Citrus limetta Peel Oil (Paperback)
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The essential peel oil of Citrus limetta var. Mitha (Sweet lime)
extracted by steam distillation was assessed for chemical
constituents and antimicrobial activity. Gas chromatographic
analysis identified 17 constituents among which Limonene (95.98 %)
was found as major component followed by camphene (1.79 %), while
the remaining terpenes were less than 1%. The results of
antimicrobial activity of essential oil tested by disc diffusion
method, against different against bacteria and fungi showed that it
exhibited maximum zone of inhibition against Bacillus cereus
(31.0mm) and Bacillus subtilis (29mm) followed by Staphylococcus
aureus (25.3mm), whereas the minimum zone of inhibition was shown
by Fusarium oxysporum (11mm) after 48 hours of incubation at their
respective temperature (370C for bacteria and 250C for fungi). The
inhibition zones, measured after 48 hours and 96 hours, showed that
it was active against all tested bacteria and fungi. The results of
our study showed that essential oil of Citrus limetta var. Mitha
peel can be an effective medicine against different pathogenic
microbes.
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