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Enzyme Stabilization and Immobilization - Methods and Protocols (Hardcover, 2nd ed. 2017)
Loot Price: R5,423
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Enzyme Stabilization and Immobilization - Methods and Protocols (Hardcover, 2nd ed. 2017)
Series: Methods in Molecular Biology, 1504
Expected to ship within 10 - 15 working days
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This volume introduces the reader to the field of enzyme
stabilization and the different theories of enzyme stabilization,
including the use of immobilization as a stabilization technique.
The first part of the book focuses on protocols for enzyme
stabilization in solutions including liposome formation, micelle
introduction, crosslinking, and additives. The second part of the
book discusses protocols for enzyme stabilization during enzyme
immobilization, including common techniques like sol-gel
encapsulation, polymer encapsulation, and single enzyme
nanoparticle formation. Protocols for a variety of enzymes are
shown, but the enzymes are chosen as examples to show that these
protocols can be used for both enzymes of biological importance, as
well as enzymes of industrial importance. The final part details
spectroscopic protocols, methods, and assays for studying the
effectiveness of the enzyme stabilization and immobilization
strategies. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
thorough, Enzyme Stabilization and Immobilization: Methods and
Protocols, Second Edition provides molecular biologists,
biochemists, and biomedical and biochemical engineers with the
state-of-the-art technical information required to effectively
stabilize their enzyme of interest in a variety of environments
(i.e., harsh temperature, pH, or solvent conditions).
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