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Books > Science & Mathematics > Biology, life sciences > Molecular biology
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Natural Killer Cells - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2016)
Loot Price: R4,896
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Natural Killer Cells - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2016)
Series: Methods in Molecular Biology, 1441
Expected to ship within 10 - 15 working days
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This volume contains collection of Natural Killer Cell
methodologies relevant for both basic and translational research.
These methodologies present new developments in the natural killer
(NK) cell field, such as understanding the influence of NK cells
metabolism on its function, identifying complexity of NK cell
subsets through mass cytometry, and determining the emergence of
memory NK cells in murine model of MCMV infection. Methods that
study NK cell migration and cytotoxicity through endpoint analysis
or live single cell imaging are also discussed. Chapters also
describe methods pertaining to translational application of NK
cells, such as ex vivo expansion of NK cells on K562 cell lines
genetically modified to express either membrane bound IL-15 or
membrane bound IL-21, large scale NK cell culture, current
techniques for engineering NK cells to express chimeric antigen
receptors or chemokine receptors using retroviral vectors,
electroporation of mRNA, and the natural phenomenon of
trogocytosis. Written in the highly successful Methods in Molecular
Biology series format, these chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Cutting
edge and thorough, Natural Killer Cells: Methods and Protocols is a
valuable resource for researchers who not only want to understand
mechanisms that govern NK cell behavior and diversity, but also for
those who want to understand how to systematically evaluate NK
cells for adoptive immunotherapy applications.
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