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Books > Medicine > Clinical & internal medicine > Diseases & disorders > Infectious & contagious diseases
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Mycobacterium Tuberculosis Protocols (Paperback, Softcover reprint of hardcover 1st ed. 2001)
Loot Price: R2,684
Discovery Miles 26 840
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Mycobacterium Tuberculosis Protocols (Paperback, Softcover reprint of hardcover 1st ed. 2001)
Series: Methods in Molecular Medicine, 54
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The aim of this book is to provide detailed protocols for studying
the molecular biology of the pathogen Mycobacterium tuberculosis,
and its int- actions with host cells. As established mycobacterial
laboratories move - wards exploiting the genome, and laboratories
with expertise in other fields apply them to mycobacteria, both
traditional and novel methodologies need to be reviewed. Thus the
chapters in Mycobacterium tuberculosis Protocols range from
perspectives on storage of strains and safety issues to the
application of the latest functional genomics technologies. The
last few years have been remarkable ones for research into M.
tuber- losis. The most important landmark by far has been the
completion of the genome sequence of the widely studied H37Rv
strain (1). We can now predict every protein and RNA molecule made
by the pathogen. This information is or will soon be enriched by
the addition of genome sequences of other strains from the M.
tuberculosis complex: a second strain of M. tuberculosis, My-
bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable
comparative data will also be provided by the genome sequences of
Mycobacterium leprae, Mycobacterium avium, and Streptomyces
coelicolor. Another recent milestone for M. tuberculosis has been
the development of efficient mutagenesis me- odologies, the lack of
which has been a major handicap in functional studies.
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