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Nonisotopic Immunoassay (Paperback, Softcover reprint of the original 1st ed. 1988)
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Discovery Miles 16 410
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Nonisotopic Immunoassay (Paperback, Softcover reprint of the original 1st ed. 1988)
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The basis of all immunoassays is the interaction of antibodies with
antigens. The most widely used immunoassay technique is
radioimmunoassay (RIA) which was first developed by Yalow and
Berson in 1959. The principle of RIA is elegantly simple. It
utilizes a competitve binding reaction between analytes and a
radio-labeled analog of the analytes (the tracer) for anti-analyte
antibodies. In addition to its exquisite specificity, extraordinary
sensitivity, good accuracy and precision, ease and rapidity of
assay and simplicity of assay development, the applicability of RIA
to a wide variety of substances has made it one of the most
powerful and versatile analytical methods of the 20th century and
beyond. Millions of RIA's are being performed annually on clinical,
biological and environmental samples in licensed laboratories. In
order to expand the use of RIA beyond the confines of these
laboratories to areas like physician's offices, patients' homes,
economically less developed countries, agricultural fields, large
scale and continuing screening tests for infectious diseases, it
has become necessary to develop non-isotopic labels. Indeed the
last fifteen years have seen the development of a great number of
ingenious non-isotopic labels in immunoassay so that a whole new
industry capitalizing on the potential market for non isotopic
immunoassays has appeared. It is the purpose of this volume to
present in depth, state-of-the-art reviews on techniques used in
non-isotopic immunoassays. Topics covered include: (1)
Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3)
Immunoassay at liquid-solid interface; (4) Membrane immunoassay and
(5) "Particle"-mediated immunoassay."
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