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Books > Science & Mathematics > Chemistry > Analytical chemistry > Qualitative analytical chemistry > Chromatography
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High-Temperature Liquid Chromatography - A User's Guide for Method Development (Hardcover, Edition.)
Loot Price: R4,135
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High-Temperature Liquid Chromatography - A User's Guide for Method Development (Hardcover, Edition.)
Series: RSC Chromatography Monographs, Volume 13
Expected to ship within 12 - 17 working days
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High-temperature liquid chromatography has attracted much interest
in recent years but has not yet recognized its full potential in
the chromatographic community. There is a widespread reluctance in
industry to use temperature to speed up the separation process,
influence the selectivity of a separation or implement novel
detection techniques. However, the technology has now matured and
could revolutionize chromatography as we see it today. Better
equipment, such as heating systems able to generate faster heating
rates, is becoming more readily available. Also, columns based on
silica gel, which can withstand higher temperatures for an extended
period, are now being introduced. Nevertheless, further
technological and methodical efforts are needed to establish the
method in a regulated environment like the pharmaceutical industry.
This is the only text to cover all the practical aspects, as well
as the underlying theoretical principles, of setting up an HPLC
system for high temperature operation. It is not intended solely
for academics but will also benefit the researcher interested in
more practical considerations. The author is a recognized expert
and has conducted several studies with partners from industry to
validate the method. Many real examples from these studies have
been included in the book. The aim is to support practitioners in
the creation of their own protocols without the need to rely solely
on trial and error. The book starts with a brief definition of high
temperature liquid chromatography before going on to cover: system
set up; the heating system; mobile phase considerations; suitable
stationary phases; method development using temperature
programming; analyte stability, and special hyphenation techniques
using superheated water as a mobile phase. In each chapter,
experimental data is used to illustrate the main statements and the
advantages over conventional HPLC are evaluated. The book concludes
with a critical outlook on further developments and applications
underlining the necessary advances needed to make high temperature
HPLC more robust.
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