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Books > Science & Mathematics > Chemistry > Analytical chemistry > Qualitative analytical chemistry > Chromatography
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High-Performance Liquid Chromatography (HPLC) - Principles, Practices & Procedures (Hardcover)
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High-Performance Liquid Chromatography (HPLC) - Principles, Practices & Procedures (Hardcover)
Expected to ship within 12 - 17 working days
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High-performance liquid chromatography (HPLC) has emerged as the
most powerful and versatile separation and analytical method. This
book covers not only the conventional HPLC techniques but also the
new developments, novel separation modes, column technology, as
well as procedures and practices, particularly the advanced
applications of HPLC in the fields of pharmaceutical, clinical,
bioanalytical and food sciences. An in-depth discussion of
separation mechanisms and characteristics of stationary and mobile
phases is well-blended with practical method development and
validation. Practical and pragmatic approaches and actual examples
for rapid and effective development of fast, selective, accurate
and reliable HPLC methods are provided. This book consists of
eleven chapters. The first chapter explains the basic principles of
hydrophilic interaction liquid chromatography (HILIC), an appealing
alternative HPLC separation mode for separating polar compounds
along with the properties of HILIC stationary and mobile phases.
The technical aspects and the applications as well as actual HILIC
method development are illustrated. The second chapter describes
recent progress in HPLC column technology. The advantages and
disadvantages of sub-2 m porous particles, solid core particles and
monolithic columns over the conventional columns are discussed in
terms of the separation efficiency, speed, resolution and
applications. Chapters Three to Eleven are devoted to advanced
applications of modern HPLC including RP-HPLC, NP-HPLC, Ion
chromatography, HILIC, LC-MS/MS in pharmaceuticals, clinical,
biomarkers, dietary supplements, food analysis and dye examination
in archeological textiles.
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