|
Showing 1 - 8 of
8 matches in All Departments
This book encompasses the full breadth of the super-resolution
imaging field, representing modern techniques that exceed the
traditional diffraction limit, thereby opening up new applications
in biomedicine. It shows readers how to use the new tools to
increase resolution in sub-nanometer-scale images of living cells
and tissue, which leads to new information about molecules,
pathways and dynamics. The book highlights the advantages and
disadvantages of the techniques, and gives state-of-the-art
examples of applications using microscopes currently available on
the market. It covers key techniques such as stimulated emission
depletion (STED), structured illumination microscopy (SSIM),
photoactivated localization microscopy (PALM), and stochastic
optical reconstruction microscopy (STORM). It will be a useful
reference for biomedical researchers who want to work with
super-resolution imaging, learn the proper technique for their
application, and simultaneously obtain a solid footing in other
techniques.
This book encompasses the full breadth of the super-resolution
imaging field, representing modern techniques that exceed the
traditional diffraction limit, thereby opening up new applications
in biomedicine. It shows readers how to use the new tools to
increase resolution in sub-nanometer-scale images of living cells
and tissue, which leads to new information about molecules,
pathways and dynamics. The book highlights the advantages and
disadvantages of the techniques, and gives state-of-the-art
examples of applications using microscopes currently available on
the market. It covers key techniques such as stimulated emission
depletion (STED), structured illumination microscopy (SSIM),
photoactivated localization microscopy (PALM), and stochastic
optical reconstruction microscopy (STORM). It will be a useful
reference for biomedical researchers who want to work with
super-resolution imaging, learn the proper technique for their
application, and simultaneously obtain a solid footing in other
techniques.
This book starts at an introductory level and leads reader to the
most advanced topics in fluorescence imaging and super-resolution
techniques that have enabled new developments such as
nanobioimaging, multiphoton microscopy, nanometrology and
nanosensors. The interdisciplinary subject of fluorescence
microscopy and imaging requires complete knowledge of imaging
optics and molecular physics. So, this book approaches the subject
by introducing optical imaging concepts before going in more depth
about advanced imaging systems and their applications.
Additionally, molecular orbital theory is the important basis to
present molecular physics and gain a complete understanding of
light-matter interaction at the geometrical focus. The two
disciplines have some overlap since light controls the molecular
states of molecules and conversely, molecular states control the
emitted light. These two mechanisms together determine essential
imaging factors such as, molecular cross-section, Stoke shift,
emission and absorption spectra, quantum yield, signal-to-noise
ratio, Forster resonance energy transfer (FRET), fluorescence
recovery after photobleaching (FRAP) and fluorescence lifetime.
These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with
basics of imaging optics and its applications. The advanced part
takes care of several imaging techniques and related
instrumentation that are developed in the last decade pointing
towards far-field diffraction unlimited imaging.
In the last decade, fluorescence microscopy has evolved from a
classical "retrospective" microscopy approach into an advanced
imaging technique that allows the observation of cellular
activities in living cells with increased resolution and
dimensions. A bright new future has arrived as the nano era has
placed a whole new array of tools in the hands of biophysicists who
are keen to go deeper into the intricacies of how biological
systems work. Following an introduction to the complex world of
optical microscopy, this book covers topics such as the concept of
white confocal, nonlinear optical microscopy, fluctuation
spectroscopies, site-specific labeling of proteins in living cells,
imaging molecular physiology using nanosensors, measuring molecular
dynamics, muscle braking and stem cell differentiation.
"Alberto Diaspro has been choreographing light's dance for over 20
years, and in Nanoscopy and Multidimensional Optical Fluorescence
Microscopy, he has assembled a diverse group of experts to explain
the methods they use to coax light to reveal biology's secrets." -
From the Foreword by Daniel Evanko, editor, Nature Methods
Nanoscopy and Multidimensional Optical Fluorescence Microscopy
demonstrates that the boundaries between sciences do blur at the
bottom, especially those that might separate the optical work of
physicists and the cellular work of microbiologists. In 18 chapters
written by pioneering researchers, this work offers the first
comprehensive and current documentation of the cutting-edge
research being accomplished in a wide range of photonic devices
with revolutionary application. The highlight of the book is its
coverage of optical nanoscopy and super-resolution microscopy. The
rapid advances in this area over the past few years offer
researchers in both photonics and molecular biologya wealth of
accomplishment upon which they can build. Offering a complete
treatment of this emerging field, this volume: Describes how
scientists have exploited the properties of light and its
fluorophore partners to overcome the resolution limit of
conventional light microscopy Delves into recent ways to minimize
the photobleaching that has long hampered many methods including
those that have the potential to capture previously unobtainable
information on the movements of single molecules Discusses the
principles, benefits, and implementation of fluorescence
correlation spectroscopy and related methods, which simplifies
analysis by limiting light to stationary focal points in a sample
Considers the most basic as well as emerging methods for improving
three-dimensional optical sectioning microscopy Reviews the basics
of FRET (
"Alberto Diaspro has been choreographing light's dance for over 20
years, and in Nanoscopy and Multidimensional Optical Fluorescence
Microscopy, he has assembled a diverse group of experts to explain
the methods they use to coax light to reveal biology's secrets." -
From the Foreword by Daniel Evanko, editor, Nature Methods
Nanoscopy and Multidimensional Optical Fluorescence Microscopy
demonstrates that the boundaries between sciences do blur at the
bottom, especially those that might separate the optical work of
physicists and the cellular work of microbiologists. In 18 chapters
written by pioneering researchers, this work offers the first
comprehensive and current documentation of the cutting-edge
research being accomplished in a wide range of photonic devices
with revolutionary application. The highlight of the book is its
coverage of optical nanoscopy and super-resolution microscopy. The
rapid advances in this area over the past few years offer
researchers in both photonics and molecular biologya wealth of
accomplishment upon which they can build. Offering a complete
treatment of this emerging field, this volume: * Describes how
scientists have exploited the properties of light and its
fluorophore partners to overcome the resolution limit of
conventional light microscopy * Delves into recent ways to minimize
the photobleaching that has long hampered many methods including
those that have the potential to capture previously unobtainable
information on the movements of single molecules * Discusses the
principles, benefits, and implementation of fluorescence
correlation spectroscopy and related methods, which simplifies
analysis by limiting light to stationary focal points in a sample *
Considers the most basic as well as emerging methods for improving
three-dimensional optical sectioning microscopy * Reviews the
basics of FRET (fluorescence resonance energy transfer) and
considers its new use for investigating protein comple
1. A Curious premise "My grandma was a beautiful woman...". This
chapter tells about the motivation to decide to do research in life
and why with the optical microscope. 2. Just observe! The optical
microscope to observe living systems, from organs to proteins. The
challenge from its invention to "tomorrow" to decipher cancer and
neurological disorders. 3.The colours of the rainbow We all live
under the rainbow, colours are delivering the energy needed to
explore the living by watching. 4. The sharpener of the light When
a curved piece of glass meets the light allows to see those fine
dietails hidden to the eyes. 5. A three-dimensional world
Flatlandia is a novel, the real world is developed along three
spatial dimensions and the optical microscope can produce
three-dimensional animated "postcard" by simply changing the lens
focus when observing around. 6. Modern times: the space and time of
observations Time is the fourth dimension that increases the budget
of information at our disposal to understand what's going on at
different time time and space scales. 7. Two photon are better than
one Quantum mechanics allows to start a joyful revolution in
optical microscopy with relevant implicantions in medicine and
biology. Two photon is a unique entity. 8. Super eyes to see beyond
physical limits Laws of physics limit the perfomances of the light
microscope. No doubts. The image reconstrution channel has no
limits if you are able to add information and the optical
microscope an unlimited super power to visualize details. 9.
Without a net Now is time to remove the net. We are skilled enough.
So lets control the shape of light to get information without
fluorescent labes. 10. The liquid microscope of the future
Illumination produces multiple messages tuning across time and
space scales and artificial intelligence can merge them to
deciphering nature. Liquid tunable microscopy could provide the
opportunity to see things differently and to change our point of
view, abandoning the obsession of representing the "real world" we
have in mind when forming an image. Lets see further! 11. Pop
microscopy "Grown-ups never understand anything on their own, and
it is tiring for children to always have to give them explanations.
"We use nice images to bring you to instruments and applications
like in a pop song that people whistle in the shower. 12.
Acknowledgments It is a love narration in the love story between a
curved piece of glass and the rainbow.
In the last decade, fluorescence microscopy has evolved from a
classical "retrospective" microscopy approach into an advanced
imaging technique that allows the observation of cellular
activities in living cells with increased resolution and
dimensions. A bright new future has arrived as the nano era has
placed a whole new array of tools in the hands of biophysicists who
are keen to go deeper into the intricacies of how biological
systems work. Following an introduction to the complex world of
optical microscopy, this book covers topics such as the concept of
white confocal, nonlinear optical microscopy, fluctuation
spectroscopies, site-specific labeling of proteins in living cells,
imaging molecular physiology using nanosensors, measuring molecular
dynamics, muscle braking and stem cell differentiation. Content
Level Research
|
|