Cultivation of malaria parasites is an important tool for the understanding of parasite biology, biochemistry, molecular biology, immunology, and pharmacology. Malaria represents the world's greatest public health problem in terms of number of people affected, levels of morbidity and mortality. The protozoan malaria parasites (Plasmodium spp.) are transmitted by infected female mosquitoes when feeding on blood. Parasites soon enter liver cells, and after several days of multiplication, are released into the bloodstream where further cycles of asexual reproduction occur, giving rise to the clinical symptoms of malaria. Some erythrocytic parasites will differentiate into presexual forms (gametocytes), which when taken up by mosquitoes in further blood meals, mature into gametes and undergo a sexual cycle Malaria is one of the most important infectious diseases affecting humans particularly in developing countries. P. falciparum an apicomplexan protozoan parasite is the causative agent of the most lethal form of human malaria.

Malaria is infectious vector borne parasitic and Plasmodium carried by the vector mosquitoes, which belong to genus Anopheles. The first anti-malarial developed later is chloroquine, primaquine, proquanil, pyrimethamine, metloquine etc. The aim of this study is to test the in-vitro antiplasmodial effect of extract and different fractions of Datura innoxia (leaves) and Diospyros melanoxylan (bark). D.innoxia (leaves) and D.melanoxylon. (barks), are collected from the lower forest of uttarakhand which is identified by the local people.The anti-malarial activity of the extract was discernible as number of parasite in mature schizont form in the total of 200 parasites. The crude extract (both water and ethanol) of D. innoxia and D. melanoxylon tested in-vitro had show antimalarial activity. The result of this preliminary study is very much encouraging. Natural products are important source of biologically active compounds and have potential for the development of novel antimalarial drugs. The eradication of the disease has been hampered by the emergence and spread of multi drug resistant malarial parasites, especially P.falciparum strains resistant to many antimalarial drugs."