The issue of male germ line mutagenesis and the effects on developmental defects in the next generation has become increasingly high profile over recent years. Mutations are thought to be becoming more prevalent as a result of: exposure to chemicals in the environment, anti cancer regimes that use genotoxic agents and assisted conception techniques. In addition to the increasing frequency of mutations in the general population, attention is also being given to the effects of epigenetic events on future generations. Male-mediated Developmental Toxicity discusses these issues comprehensively and includes further analysis on the fundamental mechanisms of mutations. With both clinical and experimental sections, written by leading experts in the field, this book will appeal to both medical practitioners and researchers.

The vast number of chemicals existing or being added into the environment, have globally aroused great concern regarding their adverse effects in human population. Development and validation of sensitive and better test systems which can assess the adverse effects of chemicals at an early stage for intervention strategies to be implemented in time is currently in progress. This book documents the latest research and showcases the versatile, state-of-the-art technique - the Comet assay - in the field of modern toxicology. The assay is a simple, sensitive rapid and visual technique for the quantitative and qualitative assessment of DNA damage in single cells. The Comet Assay in Toxicology is the first book of its kind to be devoted exclusively to the Comet assay and its applications as an important tool in modern toxicology. This multi-author book will serve as both a reference and a guide to investigations in the biomedical, biochemical and pharmaceutical sciences. Written by investigators from the fields of genetic toxicology and human epidemiology, the authors have first-hand knowledge from their chosen sub-specialities and are active contributors to the peer-reviewed scientific literature. The book is divided into five major sections, reflecting the range of interest in the exploitation of this assay. The book's scope begins with an introduction section reviewing its genesis for those new to the technique and the current knowledge of the various fields in which it finds wide acceptance. This section sets the scene by explaining why the assay has become the most sensitive and sought after assay in modern toxicology. Next is a whole section that considers various procedures being followed to assess different types of DNA damage in various cell types and is contributed by experts in the respective fields. The third section puts together the specific applications of the assay in the diverse fields ranging from genetic toxicity testing to human monitoring, and environmental toxicology. The fourth section consists of the guidelines and recommendations for the conduct of the assay in in vitro and in vivo systems, based on the recommendations of the International Workgroups on genotoxicity test procedures. Finally, the book draws to a close with an assessment of the statistics used for the understanding of the data generated by the assay. This is a unique reference book as it provides the scientific community with the advances in Comet assay as well as its applications. It also incorporates a detailed section with instant and comprehensive information on the procedure of the assay and the latest protocols being used worldwide as well as statistical analyses to be followed. The book is aimed at students as well as scientists in the area of molecular epidemiology and genetic toxicology.

Experimental Toxicology is an important text for undergraduates, post-graduates and professionals involved with studying or teaching this often controversial subject. It addresses the basic issues concerned with the practice of experimental toxicology and discusses in detail the following topics: experimental design; biochemical issues; animal husbandry; species differences; immunological issues; carcinogenesis; reproductive approaches; statistics; genetics; in vitro and molecular approaches; risk assessment; information resources; aspects of legislation; good laboratory practice; and laboratory design. The book has been updated and revised to reflect the many changes that have taken place since the first edition was published five years ago and this 2nd Edition gives special attention to the extensive changes that have taken place in the areas of molecular, genetic and reproductive toxicology and in the knowledge regarding the multiplicity of enzymes involved in foreign compound metabolism. Experimental Toxicology is a must for newcomers to the field who wish to gain an understanding of what toxicology is all about. It will also be of interest to experienced practitioners and to professionals from other areas who need a rapid introduction to the subject.

Neurotransmitters are usually considered to be endogenous substances that are released from neurons, act on receptor sites that are typically present on membranes of postsynaptic cells and produce a functional change in the properties of the target cell. They are essential features of the nervous systems of all animals and numerous chemicals can act as neurotransmitters either intentionally (e.g. pesticides) or unintentionally (neurotoxins). The most common forms of neurotoxicity are the death of neurons, degeneration of axons, damage to glial cells and interference with the axonal membrane or neurotransmission. Important neurotoxins are found among pesticides, metals, solvents, natural substances, and industrial chemicals. Environmental chemicals may also contribute to the pathology of neurodevelopmental, neuropsychiatric, and neurodegenerative disorders. Neurotransmitters and Toxicology will be particularly appealing to toxicologists with an interest in neurotoxicology in a variety of sub-disciplines, as well as neuro-chemists interested in pathology and disease mechanisms associated with neurotoxicants.

This first volume chronicles the early stages of the outbreak and world-wide spread of SARS-Cov-2 (COVID-19) and delineates the role of several disciplines in therapeutic and control measures. Documenting the epidemiologic response from China, the clinical evaluation, pathology and intial therapeutics that were available during the first 6 months and onwards of the outbreak, this book records how the response to the pandemic was mounted and how various branches of science and research combined to rapidly expand our understanding of the disease.

Concerns about the adverse effects of chemicals present in the environment have created a need for better systems to assess their potential consequences on human health. One potential solution is the versatile and state-of-the-art Comet assay. Simple, sensitive, rapid and visual, this modern toxicological method allows quantitative and qualitative assessment of DNA damage in single cells. This assay is used in diverse fields ranging from clinical applications, human monitoring and environmental toxicology through to genetic toxicity testing. This updated and revised edition of The Comet Assay in Toxicology provides the latest information on this important tool. It addresses, in-depth, the different protocols, statistical analyses and applications used worldwide. It also includes the guidelines recommended by the Working Group on Comet Assay. The book begins with a review of the genesis of the assay for those new to the technique and goes on to explain procedures followed to assess different types of DNA damage, various applications of the assay, and guidelines for the conduct of the assay in in vitro and in vivo systems. New chapters written for this edition will provide information on the most contemporary approaches and applications, including in silico approaches, on meta-analysis of data and on the application of the Comet Assay in nanotoxicology. This book will serve as both a reference and a guide to students as well as investigators in the biomedical, biochemical and pharmaceutical sciences fields.

How do you make love to a sleep, busy or exhausted woman? There is a secret formula a guy can employ so you will want sex anytime he makes it worth you while. This books tell you how. If you would like to be ready for your man's sexual advances anytime, day or night, instead of wondering, "What is he thinking, ? Now is not a good time," you can learn a few simple secrets to help you be eager for lovemaking anytime he is ready. This booklet tells you and your man how to approach intimacy when you are tired, asleep or busy for great pleasure that both of you will love. Being open and available increases the connection and strengthens the love between you. Always in the Mood will generate an unbreakable bond for you and your man. This direct, short booklet tells how your man can approach you so that you will never turn him down.

You spend a lot of time in your home and office. These environments surround you with electronic frequencies that could be out of harmony with nature and your natural biorhythms. It has only been in the last one hundred years that human bodies have been subjected to new and constantly changing frequencies that were absent in most of our evolution. The imbalance of your indoor environment can deplete your energy, make you fatigued or even sick. You have the ability to center yourself within any environment and even bring a balanced vibration to your indoor living space. The answers to a healthier indoor setting are within this book. Using this metaphysical guide to ground yourself and your environment, you can have the energy and health you desire to feel more alive. Diana learned at an early age the power of intuition and her sensitivities to the unseen world. Though touch she was able to feel the physical pain of others beginning at age seventeen. Through extensive research and training, she has honed her intuitive skills and uses them to heal herself and others.

The quiet town of Cypress, Mississippi is turned upside down when a former citizen, Raven Sawyer, returns for a funeral. Raven's fictional novel goes viral when the town folk realize that she's the author, and soon they're thinking that her book is not fiction but truth. Every little dirty detail is revealed in her book and has the people of Cypress wagging their tongues and shaking their heads at a few of the upper-class citizens and-the Sheriff. All Raven wants is to get through the funeral and go back to New York, but Sheriff Cal Rayburn can't allow her to leave after she discovers and turns over evidence that may be connected to a double homicide that he's investigating. At least, that's the excuse he's giving her, and he'll go to any length to keep her in Cypress until he can make her believe that the affair, that she thinks he had six years ago, never happened. When more dead bodies turn up, Cal feels they're connected to the double homicide, but with the evidence he has, he can't make a connection. He's left with more questions than answers. He's told that Raven's novel may have answers to questions that could help with his investigation, but what it reveals is more than what he wants to know. Famous in a Small Town is book one of An Entering Southern Country Novel trilogy. It has murder, lies, fiction, and truth-and more twists and turns than the back roads of northwestern Mississippi. Enter at your own risk.

All orgasms are not created equally. The art of the female climax, long shrouded in mystery and elusive for many women, is made accessible in this down-to-earth, illuminating text. Author and intimacy coach, Diana Anderson presents the essential ingredients and chemistry necessary to achieve advanced orgasmic experience and deep connection with your partner. Written with the express purpose of enhancing women's sex lives and strengthening the bonds in their relationships, Deep Sex discusses the health-giving pleasures of sex in simple terms for the modern woman, engaging the reader with provocative concepts, tips and techniques to enhance love-making. Filled with specific exercises and penetrating insight, this straightforward guide to the art of sensuality sheds light on ancient, little understood concepts like Kundalini energy, describing many levels of ecstatic sexual embrace and fun ideas to practice in the bedroom. The book's unpretentious approach to the many benefits of exceptional sexual climax brings new perspectives to the potentials of invigorating orgasms and reveals fun climaxes to reach for in your love life with names like Lightning Bolt Delight, To the Moon and Back and The Long Country Mile. Deep Sex is bound to shift your appreciation for the ultimate sensual moment, enhancing your authentic expression of sensual beauty.

Vanilla Lillian Carver has had her share of disappointment and heartbreak, from a high school heart throb, to a broken engagement, and then being left at the altar. She's made up her mind, she's through with love. Well, that was her plan until the love of her life returns after disappearing twelve years earlier without even saying goodbye. Can she trust him again? Her heart says yes, but common sense says run in the other direction, because there are too many unanswered questions that she's afraid to ask. Poor Lily She's running in circles.

Behind your cervix is the deepest place that your partner can penetrate you. This hidden chamber is called your Fornix, which is Latin for "vault." Within this treasure are the keys to reaching your soul, your inner beauty and your creative power. Opening up fully to your lover -- letting go of inhibitions -- allows him to reach to this depth within you. Your reward is the best orgasms of your life and joy that extends into every corner of your world. Through this level of pleasure and bonding with your lover, you will access greater personal potential than you ever knew possible. Learn how to communicate and make love with your man to become fully aroused and open this deep part of you. Learn about the different types of orgasm and how to achieve them, including the Ten Minute Orgasm and the Lightning Bolt Delight Orgasm. Learn how to trust your man, letting him love you and really see you. When this happens you radiate far more beauty and truly blossom. Your Secret Chamber guides you to achieve this. Each chapter offers step by step "sexercises" to ensure your success. Start today to take charge of your sexual satisfaction and open to the radiance that is deep within.

When Jo Preston Lane was nineteen, she left home thinking that was the answer to her problem, but now, six years later, she must return, and who she has to face is the least of her problems. To protect her family, she must keep all she had discovered five months ago a secret. She has little time to prepare for her great escape before her worst nightmare catches up with her. She can't afford to trust anyone, as her life truly depends on it. Not the police or the FBI. Mississippi Gambler is the second in A Southern Country Novel series.

The latest attack on Thera Logan's life wasn't the first one and won't be the last. Someone wants her dead, but until she realizes that these attacks aren't just random acts of violence or mere coincidences, she leaves herself wide-open. After her divorce from Nolan Logan, she decides to move from Seattle, Washington back to her hometown of Tahlequah, Oklahoma to live with her father and try to start a new life. She is surprised to find her old childhood sweetheart, Caleb Sutherland, has moved back home too. Old sparks fly until Caleb starts asking too many questions, and then the mystery surrounding Caleb's return coinciding with hers draws her suspicion. Caleb is determined to get answers to pertinent questions that he feels only Thera can answer, and he'll go to any length to get them . . . even deception. He's not the only one asking too many questions. Her ex-husband pops in for unexpected visits, wants to reconcile, and is being far too inquisitive. Confusion fogs Thera's world. What does Caleb really want? Why the sudden interest from Nolan? And who is stalking her? What does she possess that is more important to someone than her own life? Her fears and suspicions of Caleb and Nolan's annoying persistence to reconcile put her into flight mode, but fleeing will only set into motion a deadly rollercoaster ride leading up to an event that leaves her totally helpless. Her only way back is to remember when.

After twelve years of a bad marriage, Rose Daniels makes the decision to get her life back. A decision that she will be second guessing after Mother Nature does a number on her, and the murder of a local woman has Rose's small hometown eyeing her as the suspect. Rose isn't the prime suspect, she's the only suspect. Only her faith can save her, but to find that faith, layer upon layer, of who she has become will have to be peeled away. With what she's up against, it would be so much easier to give up, but giving up would be costly. It would cost her, her son and her life.

A year ago, Jo Preston was released and sent home from a mental institution. She has only vague memories of why she had been committed. Circumstances beyond her control will cause those memories to surface, one by one. A tragedy, brought on by deceit, leaves her heartbroken and rejected. She is sent away to live with some rather peculiar relatives. Her fear of being institutionalized again has her desperate to keep her past hidden, but how long can she keep it a secret when her imagination begins playing tricks on her... or is it? A voice from her past resurfaces as well as the man who wants her secret to die with her. This is a story about a young girl's heartbreak, rejection, fear, faith, and determination. Happy Valley is the first in A Southern Country Novel series.