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This volume provides key molecular biological protocols and
experimental strategies currently employed to study the various
stages of mammary gland development. Chapters are divided into four
parts covering genetically modified mouse models that exhibit
prenatal and pubertal mammary gland phenotypes, 2D and 3D-model
culture systems, methodologies for mammary stem cell isolation, and
translational applications that provide a bridge between
experimental studies of mammary gland development and the study of
human breast cancer. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mammary Gland Development: Methods
and Protocols will be useful for researchers whose primary interest
is in mammary gland development; developmental biologists,
epithelial cell biologists, and those with an interest in molecular
mechanisms underlying breast cancer.
This volume provides key molecular biological protocols and
experimental strategies currently employed to study the various
stages of mammary gland development. Chapters are divided into four
parts covering genetically modified mouse models that exhibit
prenatal and pubertal mammary gland phenotypes, 2D and 3D-model
culture systems, methodologies for mammary stem cell isolation, and
translational applications that provide a bridge between
experimental studies of mammary gland development and the study of
human breast cancer. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mammary Gland Development: Methods
and Protocols will be useful for researchers whose primary interest
is in mammary gland development; developmental biologists,
epithelial cell biologists, and those with an interest in molecular
mechanisms underlying breast cancer.
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