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This reference book provides information on plant cytogenetics for
students, instructors, and researchers. Topics covered by
international experts include classical cytogenetics of plant
genomes; plant chromosome structure; functional, molecular
cytology; and genome dynamics. In addition, chapters are included
on several methods in plant cytogenetics, informatics, and even
laboratory exercises for aspiring or practiced instructors. The
book provides a unique combination of historical and modern subject
matter, revealing the central role of plant cytogenetics in plant
genetics and genomics as currently practiced. This breadth of
coverage, together with the inclusion of methods and instruction,
is intended to convey a deep and useful appreciation for plant
cytogenetics. We hope it will inform and inspire students,
researchers, and teachers to continue to employ plant cytogenetics
to address fundamental questions about the cytology of plant
chromosomes and genomes for years to come. Hank W. Bass is a
Professor in the Department of Biological Science at Florida State
University. James A. Birchler is a Professor in the Division of
Biological Sciences at the University of Missouri.
With an increasing human population and a decreasing amount of
arable land, creative improvements in agriculture will be a
necessity in the coming decades to maintain or improve the standard
of living. In Plant Chromosome Engineering: Methods and Protocols,
expert researchers present techniques for the modification of crops
and other plant species in order to achieve the goal of developing
the much needed novel approaches to the production of food, feed,
fuel, fiber, and pharmaceuticals. This volume examines vital topics
such as transformation procedures, chromosome painting, production
of engineered minichromosomes, gene targeting and mutagenesis, site
specific integration, gene silencing, protein expression,
chromosome sorting and analysis, protocols for generating
chromosomal rearrangements, enhancer trapping, and means of
studying chromosomes in vivo. As a part of the highly successful
Methods in Molecular Biology(TM) series, the methodological
chapters include brief introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and professional tips on
troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Plant Chromosome Engineering: Methods and Protocols
highlights the spectrum of tools currently available for modifying
plant genomes and chromosomes and provides the foundation for
crucial future developments.
With an increasing human population and a decreasing amount of
arable land, creative improvements in agriculture will be a
necessity in the coming decades to maintain or improve the standard
of living. In Plant Chromosome Engineering: Methods and Protocols,
expert researchers present techniques for the modification of crops
and other plant species in order to achieve the goal of developing
the much needed novel approaches to the production of food, feed,
fuel, fiber, and pharmaceuticals. This volume examines vital topics
such as transformation procedures, chromosome painting, production
of engineered minichromosomes, gene targeting and mutagenesis, site
specific integration, gene silencing, protein expression,
chromosome sorting and analysis, protocols for generating
chromosomal rearrangements, enhancer trapping, and means of
studying chromosomes in vivo. As a part of the highly successful
Methods in Molecular Biology (TM) series, the methodological
chapters include brief introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and professional tips on
troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Plant Chromosome Engineering: Methods and Protocols
highlights the spectrum of tools currently available for modifying
plant genomes and chromosomes and provides the foundation for
crucial future developments.
This reference book provides information on plant cytogenetics for
students, instructors, and researchers. Topics covered by
international experts include classical cytogenetics of plant
genomes; plant chromosome structure; functional, molecular
cytology; and genome dynamics. In addition, chapters are included
on several methods in plant cytogenetics, informatics, and even
laboratory exercises for aspiring or practiced instructors. The
book provides a unique combination of historical and modern subject
matter, revealing the central role of plant cytogenetics in plant
genetics and genomics as currently practiced. This breadth of
coverage, together with the inclusion of methods and instruction,
is intended to convey a deep and useful appreciation for plant
cytogenetics. We hope it will inform and inspire students,
researchers, and teachers to continue to employ plant cytogenetics
to address fundamental questions about the cytology of plant
chromosomes and genomes for years to come. Hank W. Bass is a
Professor in the Department of Biological Science at Florida State
University. James A. Birchler is a Professor in the Division of
Biological Sciences at the University of Missouri.
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