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Plant Chromosome Engineering - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
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Plant Chromosome Engineering - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
Series: Methods in Molecular Biology, 701
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With an increasing human population and a decreasing amount of
arable land, creative improvements in agriculture will be a
necessity in the coming decades to maintain or improve the standard
of living. In Plant Chromosome Engineering: Methods and Protocols,
expert researchers present techniques for the modification of crops
and other plant species in order to achieve the goal of developing
the much needed novel approaches to the production of food, feed,
fuel, fiber, and pharmaceuticals. This volume examines vital topics
such as transformation procedures, chromosome painting, production
of engineered minichromosomes, gene targeting and mutagenesis, site
specific integration, gene silencing, protein expression,
chromosome sorting and analysis, protocols for generating
chromosomal rearrangements, enhancer trapping, and means of
studying chromosomes in vivo. As a part of the highly successful
Methods in Molecular Biology (TM) series, the methodological
chapters include brief introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and professional tips on
troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Plant Chromosome Engineering: Methods and Protocols
highlights the spectrum of tools currently available for modifying
plant genomes and chromosomes and provides the foundation for
crucial future developments.
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