Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

1925. For nearly twenty years under the religious instruction of a most devout and sincere minister of the gospel, Mr. Watson reached the years of his majority well grounded in orthodoxy. But at the same time, he became interested in the study of nature, and then and since he delved delightfully in the sciences. As he learned more of the facts of nature, he learned to depend more upon natural law as the sure and safe expression of Infinite Will. His own life's experience is the basis of the line of thought presented in this book. Contents: The astronomer, physicist, chemist, biologist, geologist, paleontologist, anthropologist, psychologist speaks; voice of ethics; philosopher speaks; new religion; new revelation.

This is a new release of the original 1925 edition.

For nearly twenty years under the religious instruction of a most devout and sincere minister of the gospel, Mr. Watson reached the years of his majority well grounded in orthodoxy. But at the same time, he became interested in the study of nature, and then and since he delved delightfully in the sciences. As he learned more of the facts of nature, he learned to depend more upon natural law as the sure and safe expression of Infinite Will. His own life's experience is the basis of the line of thought presented in this book. Contents: The astronomer, physicist, chemist, biologist, geologist, paleontologist, anthropologist, psychologist speaks; voice of ethics; philosopher speaks; new religion; new revelation.