Antiviral Resistance in Plants - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2012)

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 894
Release date:	August 2016
First published:	2012
Editors:	John M. Watson • Ming-Bo Wang
Dimensions:	254 x 178mm (L x W)
Format:	Paperback
Pages:	380
Edition:	Softcover reprint of the original 1st ed. 2012
ISBN-13:	978-1-4939-5722-4
Categories:	Books > Medicine > Other branches of medicine > Pathology > Medical microbiology & virology Books > Science & Mathematics > Biology, life sciences > Botany & plant sciences > Plant pathology & diseases
LSN:	1-4939-5722-8
Barcode:	9781493957224

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