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Books > Science & Mathematics > Biology, life sciences > Botany & plant sciences > Plant pathology & diseases
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Antiviral Resistance in Plants - Methods and Protocols (Hardcover, 2012 ed.)
Loot Price: R2,967
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Antiviral Resistance in Plants - Methods and Protocols (Hardcover, 2012 ed.)
Series: Methods in Molecular Biology, 894
Expected to ship within 10 - 15 working days
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Studies related to pathogen-mediated virus resistance in plants
were instrumental in providing some of the historical observations
which ultimately led to the vital discovery of double-stranded RNA
(dsRNA)-induced gene silencing or RNA interference (RNAi), which
has since revolutionized research on plant-virus interactions. In
Antiviral Resistance in Plants: Methods and Protocols, expert
researchers in the field detail many of the methods which are now
commonly used to study the phenomenon of RNA silencing in relation
to viral infections of plants. These include methods and techniques
for the isolation and quantitative/qualitative analyses of plant
small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs)
and microRNAs (miRNAs) as well as the analysis and manipulation of
virus-induced gene silencing (VIGS) in both monocotyledonous and
dicotyledenous plants and the use of hairpin RNA (hpRNA)
transgenes. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and key
tips on troubleshooting and avoiding known pitfalls. Authoritative
and practical, Antiviral Resistance in Plants: Methods and
Protocols seeks to aid scientists in the further study of this
crucially important botanical trait.
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