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Studies related to pathogen-mediated virus resistance in plants
were instrumental in providing some of the historical observations
which ultimately led to the vital discovery of double-stranded RNA
(dsRNA)-induced gene silencing or RNA interference (RNAi), which
has since revolutionized research on plant-virus interactions. In
Antiviral Resistance in Plants: Methods and Protocols, expert
researchers in the field detail many of the methods which are now
commonly used to study the phenomenon of RNA silencing in relation
to viral infections of plants. These include methods and techniques
for the isolation and quantitative/qualitative analyses of plant
small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs)
and microRNAs (miRNAs) as well as the analysis and manipulation of
virus-induced gene silencing (VIGS) in both monocotyledonous and
dicotyledenous plants and the use of hairpin RNA (hpRNA)
transgenes. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and key
tips on troubleshooting and avoiding known pitfalls. Authoritative
and practical, Antiviral Resistance in Plants: Methods and
Protocols seeks to aid scientists in the further study of this
crucially important botanical trait.
Studies related to pathogen-mediated virus resistance in plants
were instrumental in providing some of the historical observations
which ultimately led to the vital discovery of double-stranded RNA
(dsRNA)-induced gene silencing or RNA interference (RNAi), which
has since revolutionized research on plant-virus interactions. In
Antiviral Resistance in Plants: Methods and Protocols, expert
researchers in the field detail many of the methods which are now
commonly used to study the phenomenon of RNA silencing in relation
to viral infections of plants. These include methods and techniques
for the isolation and quantitative/qualitative analyses of plant
small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs)
and microRNAs (miRNAs) as well as the analysis and manipulation of
virus-induced gene silencing (VIGS) in both monocotyledonous and
dicotyledenous plants and the use of hairpin RNA (hpRNA)
transgenes. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and key
tips on troubleshooting and avoiding known pitfalls. Authoritative
and practical, Antiviral Resistance in Plants: Methods and
Protocols seeks to aid scientists in the further study of this
crucially important botanical trait.
This is a new release of the original 1925 edition.
1925. For nearly twenty years under the religious instruction of a
most devout and sincere minister of the gospel, Mr. Watson reached
the years of his majority well grounded in orthodoxy. But at the
same time, he became interested in the study of nature, and then
and since he delved delightfully in the sciences. As he learned
more of the facts of nature, he learned to depend more upon natural
law as the sure and safe expression of Infinite Will. His own
life's experience is the basis of the line of thought presented in
this book. Contents: The astronomer, physicist, chemist, biologist,
geologist, paleontologist, anthropologist, psychologist speaks;
voice of ethics; philosopher speaks; new religion; new revelation.
For nearly twenty years under the religious instruction of a most
devout and sincere minister of the gospel, Mr. Watson reached the
years of his majority well grounded in orthodoxy. But at the same
time, he became interested in the study of nature, and then and
since he delved delightfully in the sciences. As he learned more of
the facts of nature, he learned to depend more upon natural law as
the sure and safe expression of Infinite Will. His own life's
experience is the basis of the line of thought presented in this
book. Contents: The astronomer, physicist, chemist, biologist,
geologist, paleontologist, anthropologist, psychologist speaks;
voice of ethics; philosopher speaks; new religion; new revelation.
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