This study protocol, we have investigated antimicrobial, cytotoxic, antidiarrhoeal, analgesic and CNS depressant activity of the ethanolic extracts of leaves and fruits of Dillenia indicia. In the test of antimicrobial and antibacterial activity, it had been confirmed that leaf and fruit extract have inhibitory property for the growth of micro organism and it was also established by the test of MIC. The LC50 values of Dillenia indica in brine shrimp lethality bioassay were found significant. The plant parts showed antidiarrhoeal activity which was measured by castor oil induced method and charcoal plug method. All the samples showed significant analgesic activity. In the hole cross test for CNS depressant studies, the extracts also showed a decrease in locomotion in the test animals and in the open field test, the crude ethanolic extract of leaves and roots of D. indica showed a noticeable decrease in locomotion in the test animals. In the pentobarbitone induced hypnosis test, the crude ethanolic extract of leaves of D. indica at the doses 400 mg/kg significantly induced the sleep and also prolonged the duration of sleeping time in test animals as compared to control.

Thunbergia grandiflora(Acanthaceae) has been investigated for isolation of its secondary metabolites and evaluation of biological activities of the crude extractive. One compound had been isolated, which was identified by extensive analyses of resolution NMR spectral data. The powdered leaf of T. grandiflora was extracted separately with methanol. The solid extract was then subjected to vacuum liquid chromatography (VLC). Repeated chromatographic separation and purification the leaves of Thunbergia grandiflora afforded one flavonoid named 5 hydroxy-4',6,7- trimethoxy flavone, C18H16O6. The Methanolic crude extract of Thunbergia grandiflora showed moderate analgesic activity. Out of all the samples only chloroform fraction methanolic crude extract were appeared mild potent in terms of both zone of inhibition & spectrum of activity against some gram positive, gram negative bacteria and fungi. In antidiarrhoeal test and brine shrimp lethality bioassay, hexane, carbon tetrachloride, chloroform and aqueous fractions exhibited insignificant activities whereas Carbon tetra chloride fraction showed some hypoglycemic effect at a dose of 300 mg/kg body weight compared to control group.