Cervical cancer is one of the most common malignant diseases affecting women worldwide. Studies on loss of heterozygosity have been made for PTEN gene specific microsatellite markers in malignancies like breast, ovary and lungs and the results have shown a significant association. However the role of this gene is not clearly understood in cervical cancer from Indian population. A total of 135 cervical carcinoma tissues samples were analyzed for loss of heterozygosity. DNA was isolated from the samples and their matched control specimens. Polymerase chain reaction was performed using primer specific for two intragenic markers and one marker in flanking region and further electrophoresed on 8% denaturing polyacrylamide gel. Overall, 31 out of 133(23%) informative cases showed loss of heterozygosity in at least one locus in the region examined. The percentage of loss of heterozygosity for these markers ranged from 8% to 13%. Loss of heterozygosity was more frequently detected in intragenic region than in flanking region.

The discovery of green fluorescent protein and cloning of its gene from Aequorea victoria heralded a new era of investigation in virtually every field of cell and molecular biology ranging from the study of gene expression to protein sorting, organelle structure, measurement of protein-protein interaction, and so on.Before GFP, however several other fluorescent molecules has been in use but none of them could be as potential as GFP. What makes GFP so significant is its ability to fluoresces itself once formed in comparison of other bioluminescent molecules (such as aequorin and firefly luciferase) which require the addition of other substrate before they glow. Further, it is a small protein and can be attached to the protein of interest without disturbing its proper function. Also its small size allows following the fused protein, especially in organelles like neurons.

Today the world is looking towards the efficient energy generation from renewable energy sources because of extinction of fossil fuels.Wind energy is a promising source of green energy.But the problem associated with this kind of energy id due to varying wind speeds the out put is also varying so it is not possible to integrate it to grid.Here Induction generators are dominantly used because of their advantages in variable speed torque characteristics.In this book an attempt is made to discuss different types of generators used in wind energy and different topologies for control of voltage and frequency of Self Excited induction generator are discussed and new promising methods are proposed for low cost power generation.