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Isolation, Cloning, Purification and Physical Properties of GFP (Paperback)
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Isolation, Cloning, Purification and Physical Properties of GFP (Paperback)
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The discovery of green fluorescent protein and cloning of its gene
from Aequorea victoria heralded a new era of investigation in
virtually every field of cell and molecular biology ranging from
the study of gene expression to protein sorting, organelle
structure, measurement of protein-protein interaction, and so
on.Before GFP, however several other fluorescent molecules has been
in use but none of them could be as potential as GFP. What makes
GFP so significant is its ability to fluoresces itself once formed
in comparison of other bioluminescent molecules (such as aequorin
and firefly luciferase) which require the addition of other
substrate before they glow. Further, it is a small protein and can
be attached to the protein of interest without disturbing its
proper function. Also its small size allows following the fused
protein, especially in organelles like neurons.
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