The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering.
Goes beyond the standard engineering issues covered by most books and delves into structure-function relationshipsIntegration of knowledge across all areas of antibody engineering, development, and marketingDiscusses how current and future genetic engineering of cell lines will pave the way for much higher productivity

This volume represents a comprehensive examination of the newly recognized callimico/marmoset clade, which includes the smallest anthropoid primates on earth. It will explore these diminutive primates in their entirety, with sections on phylogeny, taxonomy and functional anatomy, behavioral ecology, reproductive physiology, as well as address critical conservation issues and the need for conservation action. The topics specifically selected for this volume are pivotal for understanding the evolutionary adaptations and divergence of any primate group, and especially one as diverse and curious as this. The discoveries of new taxa over the last fifteen years along with new genetic data have transformed this group from three genera (one with only a distant relationship to the others) and five recognized species, to five closely related genera, comprising at least 22 species. This volume will be the first to synthesize data on these newly recognized taxa.

This volume is an international endeavor, bringing together primary callimico and marmoset researchers from around the globe, including Brazil and the United States as well as Greece, Italy, Switzerland, and Germany. One of the merits of this volume is that it will serve as a readily accessible work that includes the major findings of several key international researchers whose work has not been easily available to English-speaking scholars. In addition, it draws together lab and field researchers, geneticists, anatomists, and behaviorists in an integrated volume that will provide the most detailed and thorough work on either callimicos or marmosets to date. This volume will also provide a timely forum for identifying future avenues of action necessary for more fully understanding and protecting this intriguing primate radiation.

Infections caused by fungi have recently attracted the attention of both clinicians and basic researchers given the heavy burden they represent for any health system. The mortality and morbidity rates associated to mycosis are progressively rising simply because some of these diseases are still neglected by health-care workers and due to the changing sensitivity to antifungal drugs displayed by these organisms. In this book, both researchers and clinicians working in the medical mycology field explore the most recent literature about specific mycosis; placing in one concise chapter thoroughly revisions of the current knowledge on virulence factors, recognition by immune cells, immunoevasion, epidemiology, new diagnosis trends and therapeutics. This book is recommended to researchers, physicians and students interested in medical mycology.

Easy to read and use, Addiction Psychiatric Medicine: A Comprehensive Board Review provides a systematic and comprehensive review of all diseases and disorders frequently tested on addiction psychiatry and addiction medicine board examinations. This high-yield manual is divided into two sections: the first section contains short chapters covering important topics in addiction medicine and psychiatry, including alcohol, sedatives, opioids, tobacco, cannabis, and stimulants; the second section divides questions into three 50-question blocks to resemble the board exam format. This learning tool is essential for exam study in addiction psychiatry or addiction medicine and an excellent resource for interacting with patients with substance abuse disorders. Includes close to 300 multiple choice questions and detailed answer explanations Presents information on clinical concepts, etiologies of diseases, treatments, and preventive measures in a format that's easy to remember and apply, with tables and clinical points. Includes commonly missed concepts on exams for addiction psychiatry and addiction medicine to help users achieve optimal sores. Offers quick access to high-yield content critical for success on board exams, clinical clerkships, and medical practice. Enhanced eBook version included with purchase. Your enhanced eBook allows you to access the question bank, plus all of the text, figures, and references from the book on a variety of devices.

Rural-Urban Water Struggles compiles diverse analyses of rural-urban water connections, discourses, identities and struggles evolving in the context of urbanization around the world. Departing from an understanding of urbanization as a process of constant making and remaking of multi-scalar territorial interactions that extend beyond traditional city boundaries and that deeply reconfigure rural-urban hydrosocial territories and interlinkages, the chapters demonstrate the need to reconsider and trouble the rural-urban dichotomy. The contributors scrutinize how existing approaches for securing urban water supply - ranging from water transfers to payments for ecosystem services - all rely on a myriad of techniques: they are produced by, and embedded in, specific institutional and legal arrangements, actor alliances, discourses, interests and technologies entwining local, regional and global scales. The different chapters show the need to better understand on-the-ground realities, taking account of inequalities in water access and control, as well as representation and cultural-political recognition among rural and urban subjects. Rural-Urban Water Struggles will be of great use to scholars of water governance and justice, environmental justice and political ecology. This book was originally published as a special issue of Water International.

This unique volume reviews the beautiful architectures and varying mechanical actions of the set of specialized cellular proteins called molecular chaperones, which provide essential kinetic assistance to processes of protein folding and unfolding in the cell. Ranging from multisubunit ring-shaped chaperonin and Hsp100 machines that use their central cavities to bind and compartmentalize action on proteins, to machines that use other topologies of recognition - binding cellular proteins in an archway or at the surface of a 'clamp' or at the surface of a globular assembly - the structures show us the ways and means the cell has devised to assist its major effectors, proteins, to reach and maintain their unique active forms, as well as, when required, to disrupt protein structure in order to remodel or degrade. Each type of chaperone is beautifully illustrated by X-ray and EM structure determinations at near- atomic level resolution and described by a leader in the study of the respective family. The beauty of what Mother Nature has devised to accomplish essential assisting actions for proteins in vivo is fully appreciable.

The litany of alarming observations about water use and misuse is now familiar-over a billion people without access to safe drinking water; almost every major river dammed and diverted; increasing conflicts over the delivery of water in urban areas; continuing threats to water quality from agricultural inputs and industrial wastes; and the increasing variability of climate, including threats of severe droughts and flooding across locales and regions. These issues present tremendous challenges for water governance. This book focuses on three major concepts and approaches that have gained currency in policy and governance circles, both globally and regionally-scarcity and crisis, marketization and privatization, and participation. It provides a historical and contextual overview of each of these ideas as they have emerged in global and regional policy and governance circles and pairs these with in-depth case studies that examine manifestations and contestations of water governance internationally. The book interrogates ideas of water crisis and scarcity in the context of bio-physical, political, social and environmental landscapes to better understand how ideas and practices linked to scarcity and crisis take hold, and become entrenched in policy and practice. The book also investigates ideas of marketization and privatization, increasingly prominent features of water governance throughout the global South, with particular attention to the varied implementation and effects of these governance practices. The final section of the volume analyzes participatory water governance, querying the disconnects between global discourses and local realities, particularly as they intersect with the other themes of interest to the volume. Promoting a view of changing water governance that links across these themes and in relation to contemporary realities, the book is invaluable for students, researchers, advocates, and policy makers interested in water governance challenges facing the developing world.

Rural-Urban Water Struggles compiles diverse analyses of rural-urban water connections, discourses, identities and struggles evolving in the context of urbanization around the world. Departing from an understanding of urbanization as a process of constant making and remaking of multi-scalar territorial interactions that extend beyond traditional city boundaries and that deeply reconfigure rural-urban hydrosocial territories and interlinkages, the chapters demonstrate the need to reconsider and trouble the rural-urban dichotomy. The contributors scrutinize how existing approaches for securing urban water supply - ranging from water transfers to payments for ecosystem services - all rely on a myriad of techniques: they are produced by, and embedded in, specific institutional and legal arrangements, actor alliances, discourses, interests and technologies entwining local, regional and global scales. The different chapters show the need to better understand on-the-ground realities, taking account of inequalities in water access and control, as well as representation and cultural-political recognition among rural and urban subjects. Rural-Urban Water Struggles will be of great use to scholars of water governance and justice, environmental justice and political ecology. This book was originally published as a special issue of Water International.

From foraging patterns in a single tree to social interactions across a home range, how primates use space is a key question in the field of primate behavioral ecology. Drawing on the latest advances in spatial analysis tools, this book offers practical guidance on applying geographic information systems (GIS) to central questions in primatology. An initial methodological section discusses niche modelling, home range analysis and agent-based modelling, with a focus on remote data collection. Research-based chapters demonstrate how ecologists apply this technology to a suite of topics including: calculating the intensity of use of both range and travel routes, assessing the impacts of logging, mining and hunting, and informing conservation strategies.

The marmosets and callimicos are diminutive monkeys from the Amazon basin and Atlantic Coastal Forest of South America. The marmosets are the smallest anthropoid primates in the world, ranging in size from approximately 100 to 350 g (Hershkovitz 1977; Soini 1988; Ford and Davis 1992; Araujo et al. 2000); calli- cos are not much bigger, at around 350-540 g (Ford and Davis 1992; Encarnacion and Heymann 1998; Garber and Leigh 2001). Overwhelming genetic evidence, from both nuclear and mitochondrial DNA, now indicates that these taxa represent a unified clade within the callitrichid radiation of New World monkeys, a finding that was unthinkable to all but a few geneticists a decade ago (see review in Cortes- Ortiz, this volume Chap. 2). With increasing evidence that the earliest anthropoids were themselves small bodied (under the 0. 8-1 kg threshold that marks all other living anthropoids; see Ross and Kay 2004), the ecology, behavior, reproductive stresses, and anatomical adaptations of the marmosets and callimicos provide the best living models with which to assess the types of adaptations that may have characterized early anthropoids. When Anthony Rylands' Marmosets and Tamarins: Systematics, Behaviour and Ecology was published in 1993, contributions focused almost entirely on tamarins due to the scarcity of data on marmoset behavior and the almost total lack of kno- edge about the enigmatic callimicos. Fortunately, this has changed (see Fig. 1).

From foraging patterns in a single tree to social interactions across a home range, how primates use space is a key question in the field of primate behavioral ecology. Drawing on the latest advances in spatial analysis tools, this book offers practical guidance on applying geographic information systems (GIS) to central questions in primatology. An initial methodological section discusses niche modelling, home range analysis and agent-based modelling, with a focus on remote data collection. Research-based chapters demonstrate how ecologists apply this technology to a suite of topics including: calculating the intensity of use of both range and travel routes, assessing the impacts of logging, mining and hunting, and informing conservation strategies.