This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of "seed stock," standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.

This volume, Why Europe? Problems of Culture and Identity: Media, Film, Gender, Youth and Education , addresses a range of issues which underlie the notions of European identity. Among them are: what does it mean to be a European? What ideologies have shaped the political debate over the last two centuries? What place will minorities find in the Europe of the twenty-first century? What roles will women play in the future communities? Will Europe become more open to diversity, or become increasingly introspective, a 'fortress Europe'?

This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of "seed stock," standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.

This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.

This volume is a collection of the lectures and discussions at an international colloquium organized by Unesco on the theme of Science and Synthesis to mark the occasion of the 10th anniversary of the death of both Einstein and Teilhard de Chardin, also the 50th anniversary of the theory of general relativity. Despite the great gulf which lies between the work of Einstein and Teilhard de Chardin, the coincidence in the dates provided an opportunity to examine the urge towards a synthesis of the scientific and philosophical approaches which lies at the very heart of the work of these two great men. It was, indeed, their common desire for an all-embracing concept of the universe which led them both to try to construct a cosmology for the modern world. So it seemed that the best way of honoring Einstein and Teilhard de Chardin was to arrange a free discussion of the current likelihood of effecting a syn thesis of scientific knowledge which would bring together some of today's most eminent scholars, inspired by the wish to make their research more meaningful by philosophic reflection. The resulting give and take of ideas would go far beyond mere commemoration; it would bring their ideas to life by setting them against the present state of science."

Information theory and cybernetics have developed along somewhat different lines in Europe and in the U. S. A. This book is to be seen as a contribution towards bridging the gap. Anyone who seeks to apply information theory in the fields of education and psychology very soon comes up against a central diffi culty: in the form in which it was developed by Shannon information theory excludes the semantic aspect. This problem is fundamental for in education, as in psychology, the semantic aspect is the very heart of the matter. Thus, while Attneave, Miller and Quastler, among others, successfully employed the concepts and units of measurement of in formation theory in the interpretation of the findings of experimental psychology, they were obliged to restrict their work to its syntactic and statistical aspects. Before we can make use of the methods and results of information we have to solve the central problem: How can theory in actual teaching, we measure the semantic information of a verbal message? The only way to do this is by extending the theory. A special concept has been deve loped for this purpose: subjective information. In place of an objectively measurable quantity (frequency of sign sequences) we set an empirically determined one: the subjective probability with which the recipient expects a certain sign sequence."

This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.

A first time proposal writer? An experienced pro? Anyone can benefit from these tips and supportive, encouraging tone. "Strategic Grantseeking for Community-Based Organizations: Using Your Whole Brain, Whole Heart and Real Soul" will give you a fresh look at the key elements that help win grant funds.

Its approach is simple and founded on three guiding principles: Honesty, Clarity, and Relationships. It is a whole-brain, whole-heart, real-soul process that invigorates organizations, staff and proposals.

This book is based on an approach the author has used in over 40 years of experience and workshops that successfully trained over 1,000 small community groups. It tackles the challenges of creating and selling the ideas that lead to winning proposals. Although it was written for health care groups, it has been used successfully for projects in education and environmental protection. It demystifies the competition for funds and provides a simple roadmap for anyone, in any field, to follow. It also provides readers with information about how to access all of the workshop leader's planning materials, binders, PPT slides and articles - all at no cost.