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Acute lymphoblastic leukemia (ALL) is a malignant disease caused by
the abnormal growth and development of early non-granular white
blood cells, or lymphoblasts. ALL occurs predominantly in children,
peaking at 4 years of age. Interferons (IFNs) are the biological
agents involved in the antiviral responses and the inhibition of
tumor growth. In oncology, the IFNs are an important treatment
options for a number of solid tumors and hematological
malignancies. The IFN- 1gene consists of 840 bp and it lacks intron
sequences. Seventy patients suffering from Childhood Acute
Lymphoblastic Leukemia were studied and analyzed for mutation
analysis of IFN- 1. So, 58.3% of the patients suffering from ALL
were observed and compared with 41.7% normal DNA samples as
control. PCR was performed and amplified gene products were
detected on agarose gel and PAGE. Finally, SSCP was performed and
mobility shift was observed in 7 out of 70 ALL samples. We observed
that ALL disease typically develops in children ages 1-10 years old
and is more prevalent in males than females. Mutation in the IFN- 1
gene was significantly related to 1-5 years in case of association
of mutation with age.
Cancer is an un-control growth of cells. Cancer cells grow in an
abnormal fashion, crowd out the normal cells. Liver is the largest
organ inside the body; hepatocellular carcinoma is a cancer arising
from liver. Laryngeal cancer affects the larynx, which is often
called voice box, common in males. Lung cancer is the deadliest
cancer in the world, leading cause of death from cancer in men.
Multiple gene mutations are involved in cancer pathology. Type I
interferons (IFN, IFN, IFN ) are the proteins involved in cell
signaling and growth. This study was conducted to determine whether
mutation in IFN 1 plays a significant role in liver, larynx and
lung cancer. Paraffin embedded tissues of liver, larynx and lung
were used for DNA isolation. SSCP technique was used to detect
mutation. Only two samples of lung carcinoma shows the mobility
shift on SSCP gel. It was observed that people in older age have
more chances of cancer. In general population, males are more prone
to larynx and lung cancer than female. Significant values were
observed (p
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