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A simple, sensitive and validated HPLC method has been developed to
determine esomeprazole and domperidone in tablet dosage form. The
chromatographic separation was achieved on a hyperchrome C-18
column column (4.6 x 150mm, 5 particle size) analytical column
using a mixture of acetonitrile: Phosphate buffer (pH 5.0) in the
ratio of 60:40 (v/v) used as the mobile phase, at a flow rate of
1.0 ml/min and detector wavelength at 290 nm. The validation of the
proposed method was carried out for specificity, linearity,
accuracy, precision, limit of detection, limit of quantitation and
robustness. Linearity of method was found to be in concentration
range 10-50 g/ml for esomeprazole and 5-25 g/ml for domperidone
with correlation coefficient greater than 0.9999. The retention
time of domperidone and esomeprazole was found to be 2.92 and 3.91
min respectively. The method can be used for simultaneous
determination of esomeprazole and domperidone."
We carried out a QSAR study on benzotriazine derivatives &
studied 28 potent GABAA receptor ligands; derivatives of
benzotriazines, using a combination of various physicochemical,
steric, electronic and thermodynamic descriptors to determine the
quantitative correlation between binding affinity and structural
features with potent anticonvulsant activity. Correlation between
these properties and anticonvulsant activity was used to synthesize
compounds possessing potent anticonvulsant activity. Most of the
compounds showed an ability to inhibit the maximum electroshock
(MES) and pentylenetetrazole (PTZ)-induced convulsions. Compound
1A, i.e. 2-(4-Chloro-phenyl)-5-nitro-1H-benzimidazole exhibited
maximum activity.
A new, simple, accurate, precise and reproducible UV
spectrophotometric method is being developed for the simultaneous
estimation of amlodipine besylate, olmesartan medoxomil and
hydrochlorthiazide in tablet dosage form. The stock solutions were
prepared in methanol. The max for amlodipine besylate, olmesartan
medoxomil and hydrochlorthiazide were 238.5nm, 256.5nm and 271.5nm
respectively. The amlodipine besylate, olmesartan medoxomil and
hydrochlorthiazide obeyed Beer's law in concentration range of
5-25ug/ml, 6-30ug/ml and 5-25ug/ml respectively. Results of
analysis of simultaneous equation method were analyzed and
validated for various parameters according to ICH guidelines.
Every year many new drugs and newer drug combinations enter the
pharmaceutical area. Analytical methods for these new and first
timer drugs are mostly confined only to the manufacturing company.
However, availability of multiple analytical methods for the same
drug/drug combinations in their formulations is always
advantageous. Moreover, development of such methods helps in
training the analysts for skillfully handling the sophisticated
analytical instruments and the way for research approach. The
present work is also planned on similar lines. A simple, precise,
rapid and accurate Reversed-Phase High Performance Liquid
Chromatography method is developed for the simultaneous estimation
of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide
in dosage forms. Chromatography is carried out isocratically at 25
C 0.5 C on Liquid chromatographic system from Young Lin 9100
comprising of hyperchrome C-18 column (4.6 x 250mm, 5 particle
size), manual injector, YL 9110 quaternary pump and Photodiode
array YL 9160 detector connected to software YL clarity. Parameters
such as linearity, precision, accuracy, recovery and specificity
are studied as reported in the ICH guidelines.
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