This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

This volume is an update of volume 225 of MIE, "Guide to Techniques in Mouse Development," edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. During the past 17 years many new technologies or methodologies have appeared for the study of mouse development and this volume comprehensively covers these, including: new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expresion, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, rederivation of animals and use of mouse genomics.

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

This volume is Part B of an update of volume 225, "Guide to Techniques in Mouse Development," edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.

Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology. The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text: Lentivirus transgenesis o Germline modification using mouse stem cells Electroporation Applications of transposons in mouse genetics Functional genomics using transposon systems The use of DNA transposons in detecting cancer genes in mice Recombination, conditional mutagenesis and induction of tamoxifen Genetic fate mapping using recombinases Genetic screens mouse ES cells Gene trap mutagenesis Mouse mutagenesis Self- renewal and pluripotency Transgenic RNAi applications Gene knockdowns Tetracycline-controlled transcription Gene expression profiling of mouse embryos The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

This volume is the first of a 2 part update of volume 225, "Guide to Techniques in Mouse Development," edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.

Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

This series provides annual reviews of research topics in developmental biology/biochemistry, written from the perspectives of leading investigators in these fields. This volume of "Advances in Developmental Biochemistry" consists of seven chapters that review specific aspects of development in several different organisms including mollusks, flies and mice.

Cellular polarization is key to all cellular functions. Our perceptions, which are derived from our senses, depend on the proper cellular polarization of our sense organs, such as the eyes or ears. Much of this book examines the different aspects in cellular polarization and its researched role in the Drosophila, where the first planar cellular polarity (PCP) gene was discovered over 20 years ago. Topics also include: From flies to man: how we are polarized, Marking an embryo work, Cellular polarization at its functional best, Hearing and seeing your environment, and From a cell to an organ.
This series represents timely issues in developmental biology. It provides annual reviews of selected topics, written from the perspectives of leading investigators in the field of development.
* Presents many various organisms such as flies, fish, frogs and mice
* Offers over 40 exceptional illustrations
* First of its kind to include new data and detailed models on cell planar polarization