This volume provides a comprehensive description of the basic tenets of molecular biology, from mechanisms to its elaborate role in gene regulation. The initial sections cover the history of genetics and molecular biology. The book then goes on to highlight the significance of molecular approaches for all biological processes in both simple and complex cells. The volume incorporates the most recent research from this ever-evolving field. The authors have described experimental approaches wherever necessary to present evidence that has led to the development of important concepts and significant advances in molecular biology.

This new volume, Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future, looks at the application of a variety of technologies, both fundamental and advanced, that are being used for crop improvement, metabolic engineering, and the development of transgenic plants. The science of agriculture is among the oldest and most intensely studied by mankind. Human intervention has led to manipulation of plant gene structure for the use of plants for the production of bioenergy, food, textiles, among other industrial uses. A sound knowledge of enzymology as well as the various biosynthetic pathways is required to further utilize microbes as sources to provide the desired products for industrial utility. This volume provides an overview of all these aspects along with an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development, and crop plant improvement. Also discussed are the use of "white biotechnology" and "metabolic engineering" as prerequisites for a sustainable development. The importance of patenting of plant products, world food safety, and the role of several imminent organizations is also discussed. The volume provides an holistic view that makes it a valuable source of information for researchers of agriculture and biotechnology as well as agricultural engineers, environmental biologists, environmental engineers, and environmentalists. Short exercises at the end of the chapters help to make the book suitable for course work in agriculture biotechnology, genetics, biology, biotechnology, and plant science.

This volume provides a comprehensive description of the basic tenets of molecular biology, from mechanisms to its elaborate role in gene regulation. The initial sections cover the history of genetics and molecular biology. The book then goes on to highlight the significance of molecular approaches for all biological processes in both simple and complex cells. The volume incorporates the most recent research from this ever-evolving field. The authors have described experimental approaches wherever necessary to present evidence that has led to the development of important concepts and significant advances in molecular biology.

This new volume, Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future, looks at the application of a variety of technologies, both fundamental and advanced, that are being used for crop improvement, metabolic engineering, and the development of transgenic plants. The science of agriculture is among the oldest and most intensely studied by mankind. Human intervention has led to manipulation of plant gene structure for the use of plants for the production of bioenergy, food, textiles, among other industrial uses. A sound knowledge of enzymology as well as the various biosynthetic pathways is required to further utilize microbes as sources to provide the desired products for industrial utility. This volume provides an overview of all these aspects along with an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development, and crop plant improvement. Also discussed are the use of "white biotechnology" and "metabolic engineering" as prerequisites for a sustainable development. The importance of patenting of plant products, world food safety, and the role of several imminent organizations is also discussed. The volume provides an holistic view that makes it a valuable source of information for researchers of agriculture and biotechnology as well as agricultural engineers, environmental biologists, environmental engineers, and environmentalists. Short exercises at the end of the chapters help to make the book suitable for course work in agriculture biotechnology, genetics, biology, biotechnology, and plant science.

Genomics in Precision Medicine makes the people aware about the field of genomics and that of precision medicine, by taking the readers through all the details related to genomics and precision medicine. It also updates the readers about the various innovations that have taken place in the field of precision medicine and discusses the path that is to be followed further. Also discussed in the book is a review on the relation between the precision medicine and the mutations that drive it, delving on the various computational methods and conformational principles for the detection of the factors that drive cancer. It also discusses the various genetic mutations and epigenetic modifications and goes on to explore the various benefits and harms in the research on precision medicine.

Microorganisms have critical roles in the functioning of environment, structural formation, and plant interactions, both positive and negative. These roles are important in reestablishing function and biodiversity in ecosystem restoration. This book will focus on topics where microbial ecology contributes to management, restoration and sustainability of the biosphere. Discussing current microbial ecology and environmental microbiology, it will review the role of microbial communities in ecosystem services, with examples from both natural and managed ecosystems. It also highlights the untold ways in which microbial communities influence environmental quality and sustainability.

The new advances in the field of genetics have led to numerous medical breakthroughs in the past few years. Genetic Analysis: Integrating Genes and Genomes provides an insight on the advances made in human genetics in the last few years. The editor has comprehensively assembled recent developments on the use of modern genetic analytical techniques and tools for answering biological questions related to genomic screening, mutations, gene and environment interactions, comparative and molecular genomic approaches for detecting genetic components of diseases. A better understanding of the genetic basis of disease can be achieved by studying genetic diversity across human populations. The interaction of gene and environment and their effects on human-specific developmental traits, genetic influences on methylation across the human life course, interplay of genes and lifestyle, genetic basis of disease in the context of migration, exposure to toxic metals like Lead, and calculation of genetic risk of diseases in unrelated individuals have been discussed. In addition, the synergistic effects of genes involved in cancer development, fructose-1,6-bisphosphatase deficiency, vitiligo, psoriasis, severe hypertriglyceridemia, and susceptibility to mycobacterial infection are explored in some chapters. Use of small interfering RNAs (siRNA), microRNAs and SINE sequences in screening systems have also been discussed to identify genes. In one of the articles, the general framework of whole exome sequence data analysis is outlined. Finally, we also present an article on ChemProt, a publicly available compilation of chemical-protein-disease annotation resources that enables the study of a molecule from cellular to clinical levels.

Cell biology, an important area of biology is the study of the cell and its components. The chapters in this book are related to cell division, biomarkers, membrane structures and functions, a few molecular analytical techniques, and cytological techniques that are useful in modern biomedical research. The chapters are original research articles and reviews dealing with advance concepts, and principles of cell biology, cell division guiding wound repair, fundamental concepts of membrane transport, dendritic cells, transcriptomics, clinical implications of metastatic cells, cellular biomarkers of psychiatric disorders, and Keynurine pathway in stem cells. A few chapters focus on analytical techniques like gene editing or CRISPR, application of Chromogenic In Situ Hybridization (CISH) for HPV Genotyping, molecular classification of cancer with the 92-gene assay panel, a model that simultaneously captures cell motility and multibody cell-cell interactions in a confluent tissue, and phase-digital holographic microscopy as a promising imaging technique to identify cellular biomarkers of psychiatric disorders. In one of the reviews, the use of alternative substrates and genetic modifications (host cell engineering) are analyzed as tools to overcome factors limiting productivity. The main objective is to provide easier access and sufficient information to the readers to enrich their existing knowledge base.

There are 2 techniques that are commonly employed in research labs: gel electrophoresis and blotting. Following electrophoresis, separated nucleic acids and proteins can be transferred to a membrane where they are analyzed or detected. Depending on the molecule being analyzed, Southern blot refers to transfer and studying DNA, Northern blotting refers to RNA, Western blotting to proteins and eastern blotting for post-translational modifications. Given the importance of blotting in the lab, this book comes at an opportune time. The book discusses the protocols and applications of blotting-Southern, western, northern and eastern blotting. Case studies have also presented to help the reader gain insights into the practicality and applicability of blotting in today's world. The editors hope that this book will serve as a lab staple just like the aforementioned blotting techniques.

The recent incidence of Coronavirus took a toll across the world (close to 16,362 mortalities as of 25th March 2020) involves the maintenance of social distancing and frequent washing to lower the occurrence of the disease. Basic hygiene practices advocated to lower the transmission of the disease are not touching the mouth or face-basic lessons in hygiene we learned in school are reemerging as points to prevent this deadly epidemic. In this concise book, the salient concepts of hygiene have been covered with practical applications in today's life. The familiar saying "Cleanliness is next to Godliness" becomes a reality even in today's world for mindful and healthy living!

While history says the earliest recorded influenza epidemic was in 1500; we have heard of epidemics throughout history: from the 1918 Spanish flu to the current 2020 COVID-19 pandemic. This book discusses the "killer" viruses in terms of their transmission, control, and prevention. Current research has also been presented to add practicality and relevance. This is one concise book that presents data on the go to get an idea of the major epidemics and the viruses behind them.

Biotechnology for Biofuels discusses the basic concept of biofuels along with its chemistry as well as classification. It also includes four different generation of biofuels such as first, second and third generation biofuels. This book also discusses about use of biofuels for various drives, advantages and disadvantages of biofuels, applications of biotechnology to renewable fuel production and future of bio fuels in fuel production, it also provides the reader with the fundamental notion of biofuels and its major classification in order to understand the application of biofuels in our daily lives along with their advantages and disadvantages.

Gels in Life Sciences discusses in detail the use of gels in food, and its significance in research and medicine. Gels as supramolecular hydrocolloids are discussed in detail, followed by their use in fish industry. A chapter is dedicated to micromechanics in a fibrin gel, and subsequent chapters throw light on the different types of gel and getting a detailed understanding of Gel Electrophoresis Separation. The book also presents an enlightening perspective on staining of gels.

Stem Cells: The Beginning of Regenerative Medicine deals with the significance of stem cells for the manufacture of regenerative medicines along with the techniques and approaches involved. It comprises future perspective of stem cells and tissue engineering together with the opportunities and challenges associated with it. It provides the reader with fundamental knowledge of stem cell technology in order to better understand its application in the production of regenerative medicines, the use of these regenerative medicines for kidney and to deal with the challenges associated with the application of stem cells. This book also discusses engineered stem cell niche matrices for rotator cuff tendon regenerative engineering, application of nanomaterials in stem cell regenerative medicine of orthopedic surgery, regenerative therapeutic potential of adipose stromal cells in early stage diabetic retinopathy and cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine.

Established Codes of Stem Cell Biology deals with the concept of stem cells and the codes of stem cell biology. Further, it consists of the description of molecules involved in stem cell biology and certain issues and concerns in the field of stem cell biology. It provides the reader with the fundamental notion of stem cells and the field of stem cell biology so as to understand about the development of modern techniques involved in the stem cell biology and therapeutic use of stem cell differentiation. This book also discusses about testing stem cell therapy in a rat model of inflammatory bowel disease, role of bone marrow stem cells and stem cell factor in mucosal regeneration, differentiation of human breast-milk stem cells to neural stem cells and neurons, development of new technologies for stem cell research and stem cell differentiation and therapeutic use.

Chromatography: An Invaluable Tool in Research and the Industry deals with the technique of chromatography and its major applications in the field of research. It consists of recycle HPLC technique used for the purification of natural products and basic principles involved in MLC. It provides the reader with the fundamental concept of chromatography so as to understand its application in the extraction of natural products, analyze posttranslational modifications and compare an electronic nose based on ultrafast gas chromatography. This book also discusses about reminder of systems of production and chromatography-based recovery of recombinant protein biopharmaceuticals, strong cation exchange chromatography in analysis of posttranslational modifications, chromatographic removal of endotoxins, dissolution testing of single- and dual-component thyroid hormone supplements, recombinant passenger proteins can be conveniently purified by one-step affinity chromatography and surfactant modified/mediated thin-layer chromatographic systems for the analysis of amino acids.

Remote Sensing in Biology: An Overview explains various aspects of remote sensing in biology describing the benefits and uses of remote sensing in agriculture and forestry. It includes the definition of remote sensing, application of remote sensing in health, introduction of digital technology and its connection with the conservation of nature and remote sensing in ecology. Provides the reader with the insights into development of remote sensing, so as to understand the use and benefits of this technology in conserving the natural resources and ecosystem.

This book examines the current state and future prospects of cell therapy. An essential guide to the data, basic science, outcome studies and decision-making processes in blood and marrow stem cell transplantation. Stem cell research also involves complex ethical and legal considerations since they involve adult, foetal tissue and embryonic sources. The book provides a resource for students that will help them learn the basics in a detailed manner that is required for scientists and clinicians.

GTPases in Cellular Signaling studies several aspects of cellular signaling including an extensive overview of GTPases and related concepts. It includes definitions of GTPASES in Signaling, GTPASES in Prokaryotes and latest trends in the cellular signaling. Provides the reader with insights into the development of its understanding, so as to innovate technologies in this context.

Flow cytometry allows a cell population to be analysed. This book describes the technology involved. This book covers managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology. This is a most useful book which will be a welcome addition for personal use and also for laboratories in a variety of disciplines.