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Main Question: G protein coupled receptors are involved in highly
efficient and specific activation of signalling pathways. How do
GPCR signalling complexes get assembled to generate such
specificity? In order to answer this question, we need to
understand how receptors and their signalling partners are
synthesized, folded and quality-controlled in order to generate
functional proteins. Then, we need to understand how each partner
of the signalling complex is selected to join a complex, and what
makes this assembly possible. GPCRs are known to be able to
function as oligomers, what drives the assembly into oligomers and
what will be the effects of such organization on specificity and
efficacy of signal transduction. Once the receptor complexes are
assembled, they need to reach different locations in the cell; what
drives and controls the trafficking of GPCR signalling complexes.
Finally, defects in synthesis, maturation or trafficking can alter
functionality of GPCRs signalling complexes; how can we manipulate
the system to make it function normally again? Pharmacological
chaperones may just be part of the answer to this question.
This volume covers the latest techniques that study the synthesis
of melatonin, its receptor function, and its effects at the
cellular and systemic level. The chapters are organized into three
parts. Part One describes methods for the detection of melatonin
and its biological derivatives in various biological samples, the
manipulation of melatonin synthesis by the pineal gland in animals,
and the principal source of melatonin in mammals. Part Two explores
methods to measure the biological effects and consequences of
melatonin binding to high-affinity G protein-coupled receptors.
Part Three describes methods to measure the physiological effects
that are regulated by melatonin in animals, particularly in rodent
models. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
thorough, Melatonin: Methods and Protocols is a valuable resource
for any researcher interested in investigating melatonin, from its
production to its mechanisms of action and systemic effects.
Main Question: G protein coupled receptors are involved in highly
efficient and specific activation of signalling pathways. How do
GPCR signalling complexes get assembled to generate such
specificity? In order to answer this question, we need to
understand how receptors and their signalling partners are
synthesized, folded and quality-controlled in order to generate
functional proteins. Then, we need to understand how each partner
of the signalling complex is selected to join a complex, and what
makes this assembly possible. GPCRs are known to be able to
function as oligomers, what drives the assembly into oligomers and
what will be the effects of such organization on specificity and
efficacy of signal transduction. Once the receptor complexes are
assembled, they need to reach different locations in the cell; what
drives and controls the trafficking of GPCR signalling complexes.
Finally, defects in synthesis, maturation or trafficking can alter
functionality of GPCRs signalling complexes; how can we manipulate
the system to make it function normally again? Pharmacological
chaperones may just be part of the answer to this question.
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