The present book contains the simple, fast and precise stability indicating method for the extended release formulation of Diclofenac sodium using high pressure liquid chromatography (HPLC).It contains the fundamental concepts to the analytical chemistry along with the in depth knowledge of HPLC. The objective of this book was to provide the basic concepts of the analysis by HPLC to the pharmacy fraternity.

Aromatherapy is a holistic healing process for thebody and mind with the use of fragrances. The basisof this therapy lies in the essential oils containedin plant materials. These can be found in leaves, flowers, roots, seeds, bark and resin. These oils arehighly concentrated and when extracted, can either beused in a pure form or diluted and blended with otheroils to produce the required strength. Essential oils are medicinal and fragrantand travel through the blood stream reacting withhormones and enzymes. When the fragrance is inhaledthe nerve ends in the nose transmit pleasurablesignals to the brain which reacts to the positivepower of the fragrant aroma inducing pleasantmemories, restoring emotional balance and encouragingrelaxation and energisation. Many of these fragrantoils have antiseptic and antitoxic qualities andoften act as an antidote to viral infections, inflammations, aches and pains.

The calibration system provides accurate results with a minimum of effort. However, a poorly constructed system can waste valuable instrument and analyst time, and result in poor quality data. This book will provide a complete set of instructions on how to set up and evaluate any analytical system, and equipments with an emphasis on providing the highest quality results with a minimum of effort. This book provides valuable and practical information that is not currently available from any other single reference source. Students/Research scholar will learn: The fundamentals of linear regression Different options for preparing data What calibration options are available Why the correlation coefficient is not a good measure of calibration quality Simple procedures and tests for evaluating calibration data

Wound is defined simply as the disruption of the cellular and anatomic continuity of a tissue. Wound may be produced by physical, chemical, thermal, microbial or immunological insult to the tissue. Attention should be directed towards discovering an agent, which will accelerate wound healing either when it is progressing normally, when it is suppressed by various agents like corticosteroids, anti neoplastics.or non- steroidal anti-inflammatory agents. Medical treatment of wound includes administration of drugs either locally (topical) or systemically (oral or parenteral) in an attempt to aid wound repair

Progeria (also known as "Hutchinson-Gilford progeria syndrome" and "Hutchinson-Gilford syndrome") is an extremely rare, severe, genetic condition wherein symptoms resembling aspects of aging are manifested at an early age. The disease has a very low incidence and occurs in one per eight million live births.Those born with progeria typically live about thirteen years, although many have been known to live into their late teens and early twenties and rare individuals may even reach their forties. It is a genetic condition that occurs as a new mutation and is not usually inherited, although there is a uniquely inheritable form. This is in contrast to another rare but similar premature aging syndrome, dyskeratosis congenita (DKC), which is inheritable and will often be expressed multiple times in a family line.

The aim of this study was to develop a depot formulation of mirtazapine by w/o emulsification method using chitosan as controlled release biodegradable polymer. Chitosan microspheres are used to provide controlled release of mirtazapine and to improve the bioavailability of degradable substances or enhance the uptake of hydrophilic substances across the epithelial layers. The effect of various parameters, such as stirring rate, drug to chitosan ratio, chitosan concentration and glutaraldehyde concentration on entrapment efficiency and release study was studied. Stirring speed affects the size of microspheres, drug to chitosan ratio and chitosan concentration affects the entrapment efficiency and release study; glutaraldehyde concentration affects the release of the drug molecule from the microspheres.

The aim of the work is to evaluate the transdermal potential of novel vesicular carrier, ethosomes, bearing aceclofenac, Non-steroidal anti-inflammatory drugs having limited transdermal permeation. Aceclofenac loaded ethosomal carriers were prepared, optimized and characterized for vesicular shape and surface morphology, scanning electronic microscopy, vesicular size, entrapment efficiency, stability, in- vitro release study.The formulation having 3% phospholipids content and 40% ethanol showing the grater entrapment and optimal average vesicle size of formulation was determine by Malvern Zetamaster & found 0.696 m and zeta potential of formulation was -6.74 mV. The vesicular suspension was kept in sealed vials (10ml) at 4 2 C and at room temperature for 45 days no change is shown in the entrapment efficiency. The optimized ethosomal formulation showed transdermal flux (226.1 g/cm /hr) for ethanolic drug solution which is grater then that of isopropyl alcohol solution (159.0 g/cm /hr). The result advocates the potential of ethosome formulation to treat rheumatic disease where facilitated penetration of the drug into muscle and synovial fluid is desirable.