This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift assay or bioluminescence resonance energy transfer; visualization of bio-active small molecules Raman microscopy; (phospho-)proteomics and transcriptomics for mode-of-action studies, CRISPR/Cas9-based chemogenomic profiling in mammalian cells; predicting drug interactions using computational approaches; comparison of compound-induced profiles using high-content imaging or cancer cell line panels and web-based tools for polypharmacology prediction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Systems Chemical Biology: Methods and Protocols is a valuable resource for novice or expert scientists and researchers trying to initiate or continue their chemical biology studies at a systems level.

Research in multimedia and computer-based learning has entered a new phase with a focus on adapting instruction to characteristics of individual learners. Managing Cognitive Load in Adaptive Multimedia Learning provides theory- and research-based recommendations on information presentation techniques for multimedia and e-learning environments. Focusing on extensively researched principles and methodologies, this book offers readers comprehensive research and practical implications in the subject while providing concrete examples on adaptive multimedia learning.

The book explores a cognitive load perspective on instructional guidance. Cognitive load theory is focused on instructional design implications and prescriptions that flow from human cognitive architecture, and it has become one of the leading theories of instructional design. According to this theoretical perspective, the purpose of instructional guidance is to reduce learner potential cognitive overload by providing appropriate information in the right time and in a suitable format. As the learner's level of prior knowledge is considered as the main factor influencing this decision, the effect of learner prior knowledge on effectiveness of instructional methods (the expertise reversal effect in cognitive load theory) provides the basic framework for the book. The fully-guided direct instruction and minimally-guided inquiry (discovery or exploratory) learning are often discussed in instructional psychology literature as examples of approaches with opposed degrees of guidance provided to the learners. This book considers the whole range of the levels of guidance (including intermediate levels) and approaches the problem of balancing learner guidance from a cognitive load perspective. The significance of this approach is in applying our current knowledge of human cognitive architecture to develop an integrated instructional approach bringing together the best features and advantages of direct instruction and inquiry learning. Both direct instruction and inquiry learning approaches have been around for long time, and their proponents can produce evidence of their effectiveness. This evidence needs to be treated within the context of appropriate learning goals in specific instructional settings for specific types of learners. This book provides an unbiased theoretical framework for managing learner instructional guidance and working principles for selecting appropriate levels and methods of instructional guidance (e.g., sequences of exploratory problems and explicit instruction; forms and levels of embedded guidance; and adapting methodologies) optimal for learners at different levels of prior knowledge.

Written by international experts, this book explores the possibilities for the next 20 years in conducting gravitational experiments in space that would make the most of the new and much-improved existing capabilities. They start from the premise that over the next decade the gravitational physics community will benefit from dramatic improvements in many technologies critical to the tests of gravity. This volume contains a comprehensive presentation of the theory, technology, missions and projects on relativistic gravity in space.

In 1898, an Austrian microbiologist Heinrich Winterberg made a curious observation: the number of microbial cells in his samples did not match the number of colonies formed on nutrient media (Winterberg 1898). About a decade later, J. Amann qu- tified this mismatch, which turned out to be surprisingly large, with non-growing cells outnumbering the cultivable ones almost 150 times (Amann 1911). These papers signify some of the earliest steps towards the discovery of an important phenomenon known today as the Great Plate Count Anomaly (Staley and Konopka 1985). Note how early in the history of microbiology these steps were taken. Detecting the Anomaly almost certainly required the Plate. If so, then the period from 1881 to 1887, the years when Robert Koch and Petri introduced their key inventions (Koch 1881; Petri 1887), sets the earliest boundary for the discovery, which is remarkably close to the 1898 observations by H. Winterberg. Celebrating its 111th anniversary, the Great Plate Count Anomaly today is arguably the oldest unresolved microbiological phenomenon. In the years to follow, the Anomaly was repeatedly confirmed by all microb- logists who cared to compare the cell count in the inoculum to the colony count in the Petri dish (cf., Cholodny 1929; Butkevich 1932; Butkevich and Butkevich 1936). By mid-century, the remarkable difference between the two counts became a universally recognized phenomenon, acknowledged by several classics of the time (Waksman and Hotchkiss 1937; ZoBell 1946; Jannasch and Jones 1959).

Back in 1991 Sumio Iijima ?rst saw images of multi-walled carbon nanotubes in the TEM. Two years later, he and Donald Bethune synthesized the ?rst single-walled nanotubes (SWNTs). Since then, we have seen tremendous - vances in both the methods for nanotube synthesis and in the understanding of their properties. Currently, centimeter-long SWNTs can be readily grown at selected positions on a solid substrate, and large quantities of nanotubes can be produced for industrial applications. Signi?cant progress has been made in producing nearly homogeneous samples of nanotubes of only a few diameters/chiralities. It is expected that the development of techniques for the synthesis of a single type of nanotube is not far away. At the same time, physical and chemical procedures for the separation of nanotube mixtures are being demonstrated. In addition to pure nanotubes, derivatized n- otubes with attached chemical or biochemical groups are being prepared. Nanotubes acting as containers for atoms, molecules (such as the "peapods") and chemical reactions are attracting signi?cant attention. In parallel with the synthetic e?ort there has been a race to decipher the properties of these materials. It is now clear that nanotubes possess unique mechanical, electrical, thermal and optical properties. Scientists and en- neers around the world are exploring a wide range of technological appli- tionsthatmakeuseoftheseproperties.

Jerry and Elizabeth Matejka met on a boat traveling from Bohemia, now known as the Czech Republic, to Baltimore, Maryland, in 1907. To pursue their dreams in America, Jerry and Elizabeth traveled halfway around the world and joined the ranks of the idealistic, hardworking immigrants flocking to the United States in the early twentieth century. Strangers at the beginning of the voyage, the two young lovers were inseparable upon arrival in America, and they married soon after their boat docked in the Maryland harbor. The Matejkas is the remarkable true story of these two determined pioneers and their life together as they started a business and raised a family in their new homeland.

Told from the loving perspective of their daughter Slava Matejka Mowll, "The Matejkas" is a compelling family biography brought alive by vivid narrative and the imagery of beautifully preserved family photographs. From the Matejkas' early days in Baltimore to the intense drama of the Second World War, the author's memoir is a fascinating view of the life experiences of first-generation Americans.

An unforgettable true saga, "The Matejkas" is a story of persistence and triumph told against the backdrop of one of the most important eras in American history.

This is the first comprehensive study of loans and debts in Central European countries in the Middle Ages and Early Modern Period. It outlines the issues of debts and loans in the Czech lands, Poland and Hungary, with respect to the influence of Austria and Germany. It focuses on the role of loans and debts in medieval and early modern society, credit markets in these countries, the mechanism of lending and borrowing, forms of credit, availability of loans, frequency of credits dealings, range of lending business, and last, but not least, the financial relationships inside the social classes and between them. The research presented in the book is based on a wide range of resources including credit contracts and agreements, evidence of loans and debts of courts, accounting of nobility, towns, churches and guilds, merchant diaries and Jewish registers, as well as other financial records. It covers a wide range of historical disciplines including economic and financial history, social history, the history of economic thought as well as the history of everyday life. It also contains a wealth of case studies, which offer, for the first time in English, a comprehensive and representative sample of the most up-to-date Central European research on the history of loans and debts and serves as a basis for a comparison with the other parts of Europe during the same period. The book is designed primarily for postgraduates, researchers and academics in financial, economic and historical sciences but will also be a valuable resource for students of business schools.

Financial schemes for flood recovery, if properly designed and implemented, might increase flood resilience. However, options for the increase of flood resilience during the recovery phase are to a large extent overlooked and the diversity of existing schemes shows that there has been a lack of consensus on how to achieve resilient flood recovery. Financial Schemes for Resilient Flood Recovery investigates how the implementation of financial schemes (government relief subsidies, insurance schemes, buy-outs, etc.) might increase flood resilience. The chapters included in this edited volume address the following questions: Shall government relief subsidies exist when there is flood insurance in place, and, if so, how might they both be coordinated? Where (or how) to decide about build back better incentives and where to go for planned relocation programs? What is the distributional equity of financial schemes for flood recovery, and has it been sufficiently treated? The book covers different approaches to flood recovery schemes with specific intervention rationales in different countries. Empirical evidence provided clearly shows the great diversity of financial flood recovery schemes. This diversity of state-funded schemes, private-based insurance schemes, and hybrids as well as planned relocation schemes indicates a lack of a consistent and strategic approach in flood risk management and flood resilience about flood recovery. The chapters in this book were originally published in the Environmental Hazards.

This primer develops Conformal Field Theory (CFT) from scratch, whereby CFT is viewed as any conformally-invariant theory that describes a fixed point of a renormalization group flow in quantum field theory. The book is divided into four lectures: Lecture 1 addresses the physical foundations of conformal invariance, while Lecture 2 examines the constraints imposed by conformal symmetry on the correlation functions of local operators, presented using the so-called projective null cone - a procedure also known as the embedding formalism. In turn, Lecture 3 focuses on the radial quantization and the operator product expansion, while Lecture 4 offers a very brief introduction to the conformal bootstrap. Derived from course-based notes, these lectures are intended as a first point of entry to this topic for Master and PhD students alike.

Streaming data is a big deal in big data these days. As more and more businesses seek to tame the massive unbounded data sets that pervade our world, streaming systems have finally reached a level of maturity sufficient for mainstream adoption. With this practical guide, data engineers, data scientists, and developers will learn how to work with streaming data in a conceptual and platform-agnostic way. Expanded from Tyler Akidau’s popular blog posts "Streaming 101" and "Streaming 102", this book takes you from an introductory level to a nuanced understanding of the what, where, when, and how of processing real-time data streams. You’ll also dive deep into watermarks and exactly-once processing with co-authors Slava Chernyak and Reuven Lax. You’ll explore: How streaming and batch data processing patterns compare The core principles and concepts behind robust out-of-order data processing How watermarks track progress and completeness in infinite datasets How exactly-once data processing techniques ensure correctness How the concepts of streams and tables form the foundations of both batch and streaming data processing The practical motivations behind a powerful persistent state mechanism, driven by a real-world example How time-varying relations provide a link between stream processing and the world of SQL and relational algebra

In 1898, an Austrian microbiologist Heinrich Winterberg made a curious observation: the number of microbial cells in his samples did not match the number of colonies formed on nutrient media (Winterberg 1898). About a decade later, J. Amann qu- tified this mismatch, which turned out to be surprisingly large, with non-growing cells outnumbering the cultivable ones almost 150 times (Amann 1911). These papers signify some of the earliest steps towards the discovery of an important phenomenon known today as the Great Plate Count Anomaly (Staley and Konopka 1985). Note how early in the history of microbiology these steps were taken. Detecting the Anomaly almost certainly required the Plate. If so, then the period from 1881 to 1887, the years when Robert Koch and Petri introduced their key inventions (Koch 1881; Petri 1887), sets the earliest boundary for the discovery, which is remarkably close to the 1898 observations by H. Winterberg. Celebrating its 111th anniversary, the Great Plate Count Anomaly today is arguably the oldest unresolved microbiological phenomenon. In the years to follow, the Anomaly was repeatedly confirmed by all microb- logists who cared to compare the cell count in the inoculum to the colony count in the Petri dish (cf., Cholodny 1929; Butkevich 1932; Butkevich and Butkevich 1936). By mid-century, the remarkable difference between the two counts became a universally recognized phenomenon, acknowledged by several classics of the time (Waksman and Hotchkiss 1937; ZoBell 1946; Jannasch and Jones 1959).

The true story of a music editor at VICE who tried to become the coolest reporter the company had ever had -- by becoming an international drug smuggler. In 2019, music reporter Slava P, an editor for VICE media, was sentenced to nine years in prison for recruiting friends into a scheme to smuggle cocaine from the U.S. into Australia. Five of them were already in jail. Immediately, Slava P was internationally infamous. Was he a victim of pressure to commit extreme acts for the sake of a good story? A product of a drug-obsessed work environment? Or a manipulator who pushed vulnerable young people into crime? Here, Slava P tells his side of the story: what exactly happened and how the precarious, dog-eat-dog atmosphere of a media company can lead the young, the naive, and the ambitious into taking crazy risks. Bad Trips is a story about drugs, hip-hop, influencers, and glamour, set against the backdrop of one of the world's most influential news and entertainment sites, VICE. Its cast of beautiful young people and semi-famous rappers passes from the seediest apartments to the most elegant of private clubs. Slava P's chronicling of his years at this famous hotbed of excess is a piercing insight into contemporary media culture. All royalties from the sale of Bad Trips go to co-author Brian Whitney.

This book is about the Random Field Ising Model (RFIM) – a paradigmatic spin model featuring a frozen disordering field. The focus is on the second-order phase transition between the paramagnetic and ferromagnetic phases, and the associated critical exponents. The book starts by summarizing the current knowledge about the RFIM from experiments, numerical simulations and rigorous mathematical results. It then reviews the classic theoretical works from the 1970’s which suggested a property of dimensional reduction – that the RFIM critical exponents should be the same as for the ordinary, non-disordered, Ising model of lower dimensionality, and related this an emergent Parisi-Sourlas supersymmetry. As is now known, these remarkable properties only hold when the spatial dimensionality of the model is larger than a critical dimension. The book presents a method to estimate the critical dimension, using standard tools such as the replica trick and perturbative renormalization group, whose result is in agreement with the numerical simulations. Some more elementary steps in the derivations are left as exercises for the readers. This book is of interest to researchers, PhD students and advanced master students specializing in statistical field theory.

Despite many books and courses teaching Thai Massage techniques, therapeutic application remains a confusing and inaccessible area. This high-level visual manual seeks to elucidate this challenge for students and professionals in Thai Massage. The reader will learn how to apply techniques and for whom, in the correct conditions, sequence, and pace. This comprehensive book incorporates neuromuscular treatments for an array of conditions whilst guiding students on how to develop fluidity in transition from technique to technique. Relying on visual prompts such as photographs, muscle charts, and anatomical images, this is an invaluably practical resource for bodywork students and teachers.

Andrei Sinyavsky wrote Strolls with Pushkin while confined to Dubrovlag, a Soviet labor camp, smuggling the pages out a few at a time to his wife. His irreverent portrait of Pushkin outraged emigres and Soviet scholars alike, yet his "disrespect" was meant only to rescue Pushkin from the stifling cult of personality that had risen up around him. Anglophone readers who question the longstanding adoration for Pushkin felt by generations of Russians will enjoy tagging along on Sinyavsky's strolls with the great poet, discussing his life, fiction, and famously untranslatable poems. This new edition of Strolls with Pushkin also includes a later essay Sinyavsky wrote on the artist, "Journey to the River Black."

Use this practical, step-by-step guide for developers and entrepreneurs to create and run your own cryptocurrency. Author Slava Gomzin has created two cryptocurrencies and describes in this book the technology and economics of cryptocurrencies as preparation for crypto trading, investing, and other business activities. A detailed overview of special topics includes security, privacy, and usability of crypto as a mainstream payment system. Part I, Understanding Crypto, explains the technology and economic, security, and usability aspects of crypto. This is an introduction to the world of cryptography, blockchain tech, and other elements of crypto such as security, privacy, and a detailed review of payment processing. Part II, Using Crypto, provides the practical knowledge you need to dive into the crypto business such as investment, trading, and even creating your own crypto project. Part III, Creating Your Own Crypto, teaches you how to launch your own crypto project and create your own cryptocurrency. What You Will Learn Know how cryptography, Bitcoin, and other cryptos work Understand how crypto becomes money, and how crypto exchanges work Use crypto as a payment method Buy your first crypto and know what exchange you should use Be aware of the most dangerous crypto attacks and what to do about security and privacy Maintain anonymity and privacy while dealing with crypto Know how Monero (the most popular privacy-centric cryptocurrency) works Create and run your own crypto project Create your own token, both regular (fungible) and NFT (non-fungible), from selecting the platform to economics and finances Who This Book Is For Crypto inventors, entrepreneurs, developers, investors, and advisors who are thinking about creating their own cryptocurrency; traders and investors, both professional and amateur, looking to enter the crypto markets; and software architects, developers, managers, consultants, executives, and crypto enthusiasts working for merchants, banks, fintech companies, and many other businesses that have started accepting crypto payments or dealing with other aspects of crypto

Freedom to Believe is a powerful collection of philosophical and religious essays by a modern poet of distinction. It introduces a highly original and controversial thinker to the Western reader. Olga Sedakova's central philosophical thought lies in the notion of existential freedom in its association with the liberating power of the arts, especially poetry. These convictions place her firmly in the Russian and European classical cultural traditions, which, in turn, have deep roots in Christianity. Devoutly Orthodox yet fiercely independent in her thinking, Sedakova's ecumenical humanism places her in opposition to both the "new left" and modern fundamentalism. Indeed, Sedakova's "conservatism" is more genuinely new than the so-called radicalism of the postmodernists, as she castigates "old totalitarianism" and new commercialism alike, in the name of a new cultural poetics and politics.

An intensely philosophical and religious poet, Olga Sedakova writes of nature, music, and the inner, spiritual life. As one of the preservers of traditional Russian culture, she stands in stark contrast to the rampant commercialization in contemporary Russian life, instead tracing her poetic roots back to the early avant-garde movements of pre-revolutionary Russia. For that stance she endured years of censorship and silencing during the Soviet regime, her poems distributed by hand in mimeographed copies or by word of mouth. This volume introduces to an English-speaking audience an extensive selection of poems by one of Russia's most distinguished lyric poets writing today.