Present investigation developed three pathways for in vitro regeneration and mass multiplication of Acacia mangium. The nodal segments from 8-10 years old phenotypically superior plus tree were used as explants for axillary bud culture. MS medium supplemented with BAP and Kinetin in combination was the best for culture establishment and shoot proliferation from the axillary buds. Microshoots were rooted in MS medium (half strength) supplemented with IBA and acclimatization of the rooted shoots was obtained in plastic pots containing soilrite. Sixty per cent of cotyledon explants developed direct adventitious buds. Callus was induced from the cotyledon and hypocotyl explants on MS medium fortified with NAA and BAP. Genetic fidelity of micropropagated plants was examined by RAPD analysis, at every subculture. No genetic variation was detected among plantlets, hence proving genetically stable regeneration method. This protocol can be very useful for for genetic engineering and mass production of quality planting material of Mangium.

Present investigation was carried out as an effort for genetic improvement of Dalbergia sissoo. Analysis of variance revealed significant variation for all the characters under study viz., seedling height, collar diameter, dry weight of leaves, leaf area, nutritional attributes and biochemical attributes.Family no. 25 (Jassur) was found best for nitrogen content, family no. 34 (Khurian) for phosphorus content and family no, 32 (Dhuk) for potassium content; family no. 28 and 25 were superior for total sugars and total phenols content. Seedling height showed higher heritability coupled with high genetic gain. The highly significant correlation between seedling height and collar diameter, dry weight of leaves and nitrogen content suggests that these characters can also be utilized for indirect selection. Cluster analysis showed distinct clustering pattern.

An efficient in vitro plant regeneration and Agrobacterium tumefaciens mediated genetic transformation protocol was described for castor (Ricinus communis L.) using meristem as explants. Shoot apex containing apical meristems were excised from 5-7 days old in vitro grown seedlings and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins singly or in combination. Kinetin (0.50 mg/l) in combination with BAP (0.25 mg/l) produced maximum number of shoot (10.33) and shoot length (5.20 cm). For root induction, in vitro produced shoots were transferred to rooting media containing 1/2 MS basal media with NAA. In Agrobacterium tumefaciens mediated genetic transformation, Agrobacterium tumefaciens strain containing construct pBIN1F harboring nptII and cry1F gene was used. The integration of the gene was confirmed by using PCR. The transformation experiment was performed by optimizing age of the seedlings and co-cultivation duration with Agrobacterium. When the effect of the age of the seedlings and co-cultivation duration evaluated fifteen days old seedlings and co-cultivated for three days yielded the frequency of transformation 2

Genetic diversity of Karanja accessions were evaluated with Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers. In present study low level of genetic diversity was observed among the karanj genotypes. Average RPI, PIC and MI value for RAPD primers were 2.09, 0.25 and 18.06 respectively; while for ISSR marker it was 0.66, 0.08 and 4.48 respectively. Genetic diversity analysis by Isozyme marker viz., POX (peroxidase), PPO (polyphenol oxidase ), EST (esterase) and SOD(superoxide dismutase) scores total 19 isozyme loci in 30 accessions of Karanja. Highest loci was scored by PPO (6 loci) followed by POX (5 loci) while 4 loci scored by SOD and EST. Highest polymorphism and highest number of polymorphic loci was shown by PPO isozyme (83.33%) while lowest polymorphism and lowest number of polymorphic loci was shown by SOD isozyme (50 %). In morphological characterization, in terms of height, tallest tree and average longest branch was recorded by NAUK-15 and NAUK-16 respectively. Average highest number of branches and largest leaflet width was recorded by NAUK-9 while longest leaflet length recorded by NAUK-1.