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This second edition expands upon the previous volume with
additional emphasis on recent innovation in basic renal research
and has a more holistic approach on associated disorders such as
complications associated with peritoneal dialysis, ischemic acute
kidney injury, the sympathetic nervous system, and vascular
calcification. The book is divided into five parts: Part I provides
a number of in vitro, in vivo, and ex vivo models of kidney disease
and associated complications; Part II looks at recent advances in
imaging techniques; Part III covers recent developments in studying
metabolism in renal ischemia and reperfusion; Part IV addresses
study and measurement of vascular calcification; and Part V
explores analytical techniques that are both topical and of
widespread relevance to the study of experimental renal disease.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Kidney Research: Experimental Protocols, Second
Edition is a valuable collection of protocols useful to new and
experienced researchers who are interested in the field of
Nephrology.
As the expense of treating a growing number of end-stage kidney
disease patients increases, greater attention has been paid to
prevention and early treatment. The study of renal disease,
however, suffers due to the complex nature of renal anatomy and
physiology and the plethora of different cell types found in the
kidney. In Kidney Research: Experimental Protocols, top experts in
the field seek to aid researchers by providing a number of
specialized techniques developed to examine this intricate system.
Through both well-established and novel methods, this volume
explores the preparation and culture of the main cell types used to
study renal disease mechanisms, the common animal models used to
mimic the various forms of human renal disease, and specific
applications and techniques used in vivo and in vitro. Written in
the highly successful Methods in Molecular Biology (TM) series
format, the chapters contain step-by-step, readily reproducible
laboratory protocols, lists of the necessary materials and
reagents, and tips on troubleshooting and avoiding known pitfalls.
Essential and cutting-edge, Kidney Research: Experimental Protocols
delivers invaluable guidance to new and experienced laboratory
researchers delving into the sophisticated study of the kidney.
Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe
systematic and careful study of histological material. Indeed,
every internal medicine discipline has its landmark papers
describing the clinico-pathological correlations. However,
increasingly, it is molecular and cellular biology that provides
the necessary mechanistic insights. For many years, it was thought
that the two skill sets were mutually exclusive, but we hope that
this book shows that this is not necessarily so.
Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof
the book concentrates on the preparation of tissue, providing an
overview of fixation, embedding, and processing (Chapter 1), and in
Chapters 2 and 3, the required techniques for the retrieval of RNA
from histological sections. Both routine and specialist
histological staining techniques are provided in Part II. These
include pro- cols for immuno (Chapters 4-7), lectin (Chapter 8),
and hybridization (Chapter 9) histochemistry,histologicalstaining
(Chapters10and11),aswellasspecificmethods for the in situ
identification of hypoxia (Chapter 12) and apoptosis (Chapter 13).
Finally, Part III details advances in imaging (Chapters 14-16) and
image analysis (Chapter 17). It is hoped that this volume will
provide molecular biologists with the basic his- chemical
techniques and histologists with the molecular techniques to
realise the potential of their resource. We are indebted to the
authors for their generosity in sharing these protocols.
This second edition expands upon the previous volume with
additional emphasis on recent innovation in basic renal research
and has a more holistic approach on associated disorders such as
complications associated with peritoneal dialysis, ischemic acute
kidney injury, the sympathetic nervous system, and vascular
calcification. The book is divided into five parts: Part I provides
a number of in vitro, in vivo, and ex vivo models of kidney disease
and associated complications; Part II looks at recent advances in
imaging techniques; Part III covers recent developments in studying
metabolism in renal ischemia and reperfusion; Part IV addresses
study and measurement of vascular calcification; and Part V
explores analytical techniques that are both topical and of
widespread relevance to the study of experimental renal disease.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Kidney Research: Experimental Protocols, Second
Edition is a valuable collection of protocols useful to new and
experienced researchers who are interested in the field of
Nephrology.
The technique of in situ hybridization, in its various forms, has
been used routinely in many laboratories for a number of years. In
the post-genome era, gene arrays and proteomics have allowed us to
identify hitherto unknown unrecognized pathways and mechanisms.
However, rather than diminish the importance of in situ
hybridization, the now widespread use of screening te- nologies has
increased the need to temporally and spatially localize the dist-
bution of mRNA expression. Our intention, in In Situ Hybridization
Protocols is to provide ample inf- mation for novices planning to
set up the in situ hybridization technique and use it in their
laboratory for the first time, as well as giving updates of recent
developments for those laboratories where in situ hybridization
techniques are already in use. Despite its widespread significance,
in situ hybridization has retained a re- tation as one of the more
difficult and capricious molecular biological te- niques. This may
in part be because of the hybrid nature of the technique, which
often requires a mixture of molecular biological and histological
skills. The two techniques are usually taught and acquired in
different streams of biolo- cal science. The step-by-step and
detailed protocols provided in In Situ Hybridization Protocols by
researchers active in the field should make it p- sible for both
the molecular biologist with little experience of histology and the
histologist with little experience of molecular biology to use the
technique s- cessfully in their laboratories.
Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe
systematic and careful study of histological material. Indeed,
every internal medicine discipline has its landmark papers
describing the clinico-pathological correlations. However,
increasingly, it is molecular and cellular biology that provides
the necessary mechanistic insights. For many years, it was thought
that the two skill sets were mutually exclusive, but we hope that
this book shows that this is not necessarily so.
Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof
the book concentrates on the preparation of tissue, providing an
overview of fixation, embedding, and processing (Chapter 1), and in
Chapters 2 and 3, the required techniques for the retrieval of RNA
from histological sections. Both routine and specialist
histological staining techniques are provided in Part II. These
include pro- cols for immuno (Chapters 4-7), lectin (Chapter 8),
and hybridization (Chapter 9) histochemistry, histologicalstaining
(Chapters10and11), aswellasspecificmethods for the in situ
identification of hypoxia (Chapter 12) and apoptosis (Chapter 13).
Finally, Part III details advances in imaging (Chapters 14-16) and
image analysis (Chapter 17). It is hoped that this volume will
provide molecular biologists with the basic his- chemical
techniques and histologists with the molecular techniques to
realise the potential of their resource. We are indebted to the
authors for their generosity in sharing these protocol
The technique of in situ hybridization, in its various forms, has
been used routinely in many laboratories for a number of years. In
the post-genome era, gene arrays and proteomics have allowed us to
identify hitherto unknown unrecognized pathways and mechanisms.
However, rather than diminish the importance of in situ
hybridization, the now widespread use of screening te- nologies has
increased the need to temporally and spatially localize the dist-
bution of mRNA expression. Our intention, in In Situ Hybridization
Protocols is to provide ample inf- mation for novices planning to
set up the in situ hybridization technique and use it in their
laboratory for the first time, as well as giving updates of recent
developments for those laboratories where in situ hybridization
techniques are already in use. Despite its widespread significance,
in situ hybridization has retained a re- tation as one of the more
difficult and capricious molecular biological te- niques. This may
in part be because of the hybrid nature of the technique, which
often requires a mixture of molecular biological and histological
skills. The two techniques are usually taught and acquired in
different streams of biolo- cal science. The step-by-step and
detailed protocols provided in In Situ Hybridization Protocols by
researchers active in the field should make it p- sible for both
the molecular biologist with little experience of histology and the
histologist with little experience of molecular biology to use the
technique s- cessfully in their laboratories.
This third edition provides updated and new chapters on recent
innovations in basic renal research. Chapters are divided into
three sections covering in vitro, in vivo, and ex
vivo models of kidney disease, recent advances in imaging
techniques and protocols for performing analytical and functional
measurements in the kidney; techniques that are both topical and of
widespread relevance to the study of kidney biology and
disease. Written in the format of the highly
successful Methods in Molecular Biology series, each
chapter includes an introduction to the topic, lists necessary
materials and methods, includes tips on troubleshooting and known
pitfalls, and step-by-step, readily reproducible protocols. Â
Authoritative and cutting-edge, Kidney Research: Experimental
Protocols, Third Edition aims to be comprehensive guide for
researchers in the field.
As the expense of treating a growing number of end-stage kidney
disease patients increases, greater attention has been paid to
prevention and early treatment. The study of renal disease,
however, suffers due to the complex nature of renal anatomy and
physiology and the plethora of different cell types found in the
kidney. In Kidney Research: Experimental Protocols, top experts in
the field seek to aid researchers by providing a number of
specialized techniques developed to examine this intricate system.
Through both well-established and novel methods, this volume
explores the preparation and culture of the main cell types used to
study renal disease mechanisms, the common animal models used to
mimic the various forms of human renal disease, and specific
applications and techniques used in vivo and in vitro. Written in
the highly successful Methods in Molecular Biologyâ„¢ series
format, the chapters contain step-by-step, readily reproducible
laboratory protocols, lists of the necessary materials and
reagents, and tips on troubleshooting and avoiding known pitfalls.
Essential and cutting-edge, Kidney Research: Experimental Protocols
delivers invaluable guidance to new and experienced laboratory
researchers delving into the sophisticated study of the kidney.
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