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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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In Situ Hybridization Protocols (Hardcover, 3rd ed. 2006)
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In Situ Hybridization Protocols (Hardcover, 3rd ed. 2006)
Series: Methods in Molecular Biology, 326
Expected to ship within 10 - 15 working days
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The technique of in situ hybridization, in its various forms, has
been used routinely in many laboratories for a number of years. In
the post-genome era, gene arrays and proteomics have allowed us to
identify hitherto unknown unrecognized pathways and mechanisms.
However, rather than diminish the importance of in situ
hybridization, the now widespread use of screening te- nologies has
increased the need to temporally and spatially localize the dist-
bution of mRNA expression. Our intention, in In Situ Hybridization
Protocols is to provide ample inf- mation for novices planning to
set up the in situ hybridization technique and use it in their
laboratory for the first time, as well as giving updates of recent
developments for those laboratories where in situ hybridization
techniques are already in use. Despite its widespread significance,
in situ hybridization has retained a re- tation as one of the more
difficult and capricious molecular biological te- niques. This may
in part be because of the hybrid nature of the technique, which
often requires a mixture of molecular biological and histological
skills. The two techniques are usually taught and acquired in
different streams of biolo- cal science. The step-by-step and
detailed protocols provided in In Situ Hybridization Protocols by
researchers active in the field should make it p- sible for both
the molecular biologist with little experience of histology and the
histologist with little experience of molecular biology to use the
technique s- cessfully in their laboratories.
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