In Situ Hybridization Protocols (Hardcover, 3rd ed. 2006)

The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 326
Release date:	December 2005
First published:	2006
Editors:	Ian A. Darby • Tim D Hewitson
Dimensions:	235 x 155 x 25mm (L x W x T)
Format:	Hardcover
Pages:	270
Edition:	3rd ed. 2006
ISBN-13:	978-1-58829-402-9
Categories:	Books > Science & Mathematics > Biology, life sciences > Molecular biology Books > Medicine > Other branches of medicine > Pathology > Gross pathology Books > Science & Mathematics > Biology, life sciences > Cellular biology > General Promotions
LSN:	1-58829-402-1
Barcode:	9781588294029

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