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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Histology Protocols (Hardcover, 2010)
Loot Price: R3,158
Discovery Miles 31 580
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Histology Protocols (Hardcover, 2010)
Series: Methods in Molecular Biology, 611
Expected to ship within 10 - 15 working days
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Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe
systematic and careful study of histological material. Indeed,
every internal medicine discipline has its landmark papers
describing the clinico-pathological correlations. However,
increasingly, it is molecular and cellular biology that provides
the necessary mechanistic insights. For many years, it was thought
that the two skill sets were mutually exclusive, but we hope that
this book shows that this is not necessarily so.
Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof
the book concentrates on the preparation of tissue, providing an
overview of fixation, embedding, and processing (Chapter 1), and in
Chapters 2 and 3, the required techniques for the retrieval of RNA
from histological sections. Both routine and specialist
histological staining techniques are provided in Part II. These
include pro- cols for immuno (Chapters 4-7), lectin (Chapter 8),
and hybridization (Chapter 9) histochemistry, histologicalstaining
(Chapters10and11), aswellasspecificmethods for the in situ
identification of hypoxia (Chapter 12) and apoptosis (Chapter 13).
Finally, Part III details advances in imaging (Chapters 14-16) and
image analysis (Chapter 17). It is hoped that this volume will
provide molecular biologists with the basic his- chemical
techniques and histologists with the molecular techniques to
realise the potential of their resource. We are indebted to the
authors for their generosity in sharing these protocol
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