This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Protein folding and aggregation is the process by which newly synthesized proteins fold into the specific three-dimensional structures defining their biologically active states. It has always been a major focus of research in biochemistry and has often been seen as the unsolved second part of the genetic code. In the last 10 years we have witnessed a quantum leap in the research in this exciting area. Computational methods have improved to the extent of making possible to simulate the complete folding process of small proteins and the early stages of protein aggregation. Experimental methods have evolved to permit resolving fast processes of folding reactions and visualizing single molecules during folding. The findings from these novel experiments and detailed computer simulations have confirmed the main predictions of analytical theory of protein folding. In summary, protein folding research has finally acquired the status of a truly quantitative science, paving the way for more exciting developments in the near future. This unique book covers all the modern approaches and the many advances experienced in the field during the last 10 years. There is also much emphasis on computational methods and studies of protein aggregation which have really flourished in the last decade. It includes chapters in the areas that have witnessed major developments and are written by top experts including:computer simulations of folding, fast folding, single molecule spectroscopy, protein design, aggregation studies (both computational and experimental). Readers will obtain a unique perspective of the problems faced in the biophysical study of protein conformational behaviour in aqueous solution and how these problems are being solved with a multidisciplinary approach that combines theory, experiment and computer simulations. Protein Folding, Misfolding and Aggregation Classical Themes and Novel Approaches is essential reading for graduate students actively involved in protein folding research, other scientists interested in the recent progress of the field and instructors revamping the protein folding section of their biochemistry and biophysics courses.

This book contains a selection of papers accepted for presentation and discussion at ROBOT 2015: Second Iberian Robotics Conference, held in Lisbon, Portugal, November 19th-21th, 2015. ROBOT 2015 is part of a series of conferences that are a joint organization of SPR - "Sociedade Portuguesa de Robotica/ Portuguese Society for Robotics", SEIDROB - Sociedad Espanola para la Investigacion y Desarrollo de la Robotica/ Spanish Society for Research and Development in Robotics and CEA-GTRob - Grupo Tematico de Robotica/ Robotics Thematic Group. The conference organization had also the collaboration of several universities and research institutes, including: University of Minho, University of Porto, University of Lisbon, Polytechnic Institute of Porto, University of Aveiro, University of Zaragoza, University of Malaga, LIACC, INESC-TEC and LARSyS. Robot 2015 was focussed on the Robotics scientific and technological activities in the Iberian Peninsula, although open to research and delegates from other countries. The conference featured 19 special sessions, plus a main/general robotics track. The special sessions were about: Agricultural Robotics and Field Automation; Autonomous Driving and Driver Assistance Systems; Communication Aware Robotics; Environmental Robotics; Social Robotics: Intelligent and Adaptable AAL Systems; Future Industrial Robotics Systems; Legged Locomotion Robots; Rehabilitation and Assistive Robotics; Robotic Applications in Art and Architecture; Surgical Robotics; Urban Robotics; Visual Perception for Autonomous Robots; Machine Learning in Robotics; Simulation and Competitions in Robotics; Educational Robotics; Visual Maps in Robotics; Control and Planning in Aerial Robotics, the XVI edition of the Workshop on Physical Agents and a Special Session on Technological Transfer and Innovation.

This book contains a selection of papers accepted for presentation and discussion at ROBOT 2015: Second Iberian Robotics Conference, held in Lisbon, Portugal, November 19th-21th, 2015. ROBOT 2015 is part of a series of conferences that are a joint organization of SPR - "Sociedade Portuguesa de Robotica/ Portuguese Society for Robotics", SEIDROB - Sociedad Espanola para la Investigacion y Desarrollo de la Robotica/ Spanish Society for Research and Development in Robotics and CEA-GTRob - Grupo Tematico de Robotica/ Robotics Thematic Group. The conference organization had also the collaboration of several universities and research institutes, including: University of Minho, University of Porto, University of Lisbon, Polytechnic Institute of Porto, University of Aveiro, University of Zaragoza, University of Malaga, LIACC, INESC-TEC and LARSyS. Robot 2015 was focussed on the Robotics scientific and technological activities in the Iberian Peninsula, although open to research and delegates from other countries. The conference featured 19 special sessions, plus a main/general robotics track. The special sessions were about: Agricultural Robotics and Field Automation; Autonomous Driving and Driver Assistance Systems; Communication Aware Robotics; Environmental Robotics; Social Robotics: Intelligent and Adaptable AAL Systems; Future Industrial Robotics Systems; Legged Locomotion Robots; Rehabilitation and Assistive Robotics; Robotic Applications in Art and Architecture; Surgical Robotics; Urban Robotics; Visual Perception for Autonomous Robots; Machine Learning in Robotics; Simulation and Competitions in Robotics; Educational Robotics; Visual Maps in Robotics; Control and Planning in Aerial Robotics, the XVI edition of the Workshop on Physical Agents and a Special Session on Technological Transfer and Innovation.

This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.