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In an era where students suffer more than ever from fatigue and
tension, and when, because of television and other factors, their
concentration is in serious need of improvement, the subject of
Suggestopedia is more relevant than ever. Studies consistently show
that Suggestopedia and its adaptations alleviate stress and improve
focusing and memorization. Suggestopedia and Language Acquisition
examines methods for unconscious assimilation, and in particular,
Suggestopedia, its variants, its adaptations, and its background
elements.
PCR Cloning Protocols, Second Edition, updates and expands Bruce
White's best-selling PCR Cloning Protocols (1997) with the newest
procedures for DNA cloning and mutagenesis. Here the researcher
will find readily reproducible methods for all the major aspects of
PCR use, including PCR optimization, computer programs for PCR
primer design and analysis, and novel variations for cloning genes
of special characteristics or origin, with emphasis on long
distance PCR and GC-rich template amplification. Also included are
both conventional and novel enzyme-free and restriction site-free
procedures to clone PCR products into a range of vectors, as well
as state-of-the-art protocols to facilitate DNA mutagenesis and
recombination, and to clone the challenging uncharacterized DNA
flanking a known DNA fragment.
PCR Cloning Protocols, Second Edition, updates and expands Bruce
White's best-selling PCR Cloning Protocols (1997) with the newest
procedures for DNA cloning and mutagenesis. Here the researcher
will find readily reproducible methods for all the major aspects of
PCR use, including PCR optimization, computer programs for PCR
primer design and analysis, and novel variations for cloning genes
of special characteristics or origin, with emphasis on long
distance PCR and GC-rich template amplification. Also included are
both conventional and novel enzyme-free and restriction site-free
procedures to clone PCR products into a range of vectors, as well
as state-of-the-art protocols to facilitate DNA mutagenesis and
recombination, and to clone the challenging uncharacterized DNA
flanking a known DNA fragment.
First published in 1999. Routledge is an imprint of Taylor &
Francis, an informa company.
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