PCR Cloning Protocols (Paperback, Softcover reprint of hardcover 2nd ed. 2002)

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 192
Release date:	November 2010
First published:	2002
Editors:	Bing-Yuan Chen • Harry W Janes
Dimensions:	235 x 155 x 25mm (L x W x T)
Format:	Paperback
Pages:	439
Edition:	Softcover reprint of hardcover 2nd ed. 2002
ISBN-13:	978-1-61737-281-0
Categories:	Books > Reference & Interdisciplinary > Interdisciplinary studies > General Books > Science & Mathematics > Biology, life sciences > Biochemistry > General Books > Science & Mathematics > Biology, life sciences > Cellular biology > General Promotions
LSN:	1-61737-281-1
Barcode:	9781617372810

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