PCR Cloning Protocols (Hardcover, 2nd ed. 2002)

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 192
Release date:	April 2002
First published:	2002
Editors:	Bing-Yuan Chen • Harry W Janes
Dimensions:	235 x 155 x 28mm (L x W x T)
Format:	Hardcover
Pages:	439
Edition:	2nd ed. 2002
ISBN-13:	978-0-89603-969-8
Categories:	Books > Science & Mathematics > Biology, life sciences > Molecular biology Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment > General Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > General Promotions
LSN:	0-89603-969-2
Barcode:	9780896039698

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