PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Fundamentals of Combustion Processes is designed as a textbook for an upper-division undergraduate and graduate level combustion course in mechanical engineering. The authors focus on the fundamental theory of combustion and provide a simplified discussion of basic combustion parameters and processes such as thermodynamics, chemical kinetics, ignition, diffusion and pre-mixed flames. The text includes exploration of applications, example exercises, suggested homework problems and videos of laboratory demonstrations

"The Application of Biofluid Mechanics: Boundary Effects on Phoretic Motions of Colloidal Spheres" focuses on the phoretic motion behavior of various micron- to nanometer-size particles. The content of this book is divided into two parts: one on the concentration gradient-driven diffusiophoresis and osmophoresis, and one on thermocapillary motion and thermophoretic motion driven by temperature gradient. Diffusiophoresis and osmophoresis are mainly used in biomedical engineering applications, such as drug delivery, purification, and the description of the behavior of the immune system; thermocapillary motion and thermophoretic motion are applied in the field of semiconductors as well as in suspended impurities removal. The book also provides a variety of computer programming source codes compiled using Fortran for researchers' future applications.

This book is intended for chemical engineers, biomedical engineers and scientists, biophysicists and fundamental chemotaxis researchers. Dr. Po-Yuan Chen is an Assistant Professor at the Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.

Fundamentals of Combustion Processes is designed as a textbook for an upper-division undergraduate and graduate level combustion course in mechanical engineering. The authors focus on the fundamental theory of combustion and provide a simplified discussion of basic combustion parameters and processes such as thermodynamics, chemical kinetics, ignition, diffusion and pre-mixed flames. The text includes exploration of applications, example exercises, suggested homework problems and videos of laboratory demonstrations

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Immediately Sequential Bilateral Cataract Surgery (ISBCS): Global History and Methodology is a comprehensive guide for the safe and efficient performance of ISBCS. It provides an overview of the history, recommended protocols, necessary precautions, exclusion criteria, benefits and risks associated with this procedure, organizational standards, and more. Written by the most prominent ISBCS surgeons in the world, chapters recount the arguments for and against bilateral surgery that have been made throughout the ages, up to the present, and deliver achieved and proposed resolutions to all problems discussed. Topics such as ethics and medico-legal issues surrounding ISBCS are also discussed, and a chapter on the global evolution of bilateral cataract surgery provides readers with a complete overview of the development of ISBCS practices in countries from all continents.

Engineering Energy Aluminum Conductor Composite Core (ACCC) and its Application discusses this group of aluminum fibers that are characterized by their lightweight, high tensile strength, large current-carrying capacity, high temperature resistance, corrosion resistance, and small, linear expansion coefficient which make the ACCC of carbon fiber the most promising new conductor species. With the development of this technology and the reduction in product price, the ACCC of carbon fiber can play an important role in power grid construction and upgrading. The book contains a large number of practical engineering application examples and problems found in practical operation and maintenance. It is an ideal reference, providing theoretical and practical support for those in electrical engineering, aluminum conductors and overhead transmission lines.

This book included five parts: Getting Started: Selecting a Research Focus- families of children with DMD; Asking Compelling Clinical Questions- resilience in families of children with disabilities to noting family crisis meaning and resilience; Logical Inquiry through the Research Process-key components of family resilience, big factors and quality, changes in society affect family ability to cope; Review of Theory and Measurement-which measurements are easy to apply in research; and Applied, Generated, and Disseminated: list the completed dissertation Cultivating logic inquiry encourage readers to build their beliefs and abilities to actively engaged in intervention, and facilitate a change and critical appraisal. This book will provide students to know how to change study skills to accommodate the research design and improve continued academic performance. Parents, patients or teachers in any other discipline or in caring of any other chronic disease will pick up new ideas to build family relationship an to use resources for improving your quality of life by reading this book.

Through natural evolvement in thousands of years, biosurfaces have become highly adaptable to display their biological functions perfectly. Interestingly, they have developed micro-/nanostructures with gradient features to achieve smart wetting controls, such as ultra-hydrophobic water repellency in lotus leaf, directional water collection in wetted spider silk, directional adhesion in superhydrophobic butterfly wing, and fog-collecting hydrophobic/hydrophilic pattern on beetle back. These surfaces provide endless inspiration for the design and fabrication of functional interface materials with unique wettability, generating promising applications such as micro-fluidic devices, functional textiles, corrosion resistance, liquid transportation, antifogging, and water-collecting devices. In recent years there has been an exciting confluence of research areas of physics, chemistry, biology, and materials science to develop functional micro- and nanosurfaces. A kernel consists of organic materials with high/low surface energy and regular/irregular order/disorder, which can be rough/smooth and endlessly arranged and combined with various styles of micro- and nanostructures. This book introduces recent research on wettability of biological and bio-inspired surfaces. It discusses the mechanism of smart wetting controls, such as water collection/repellency on biological micro-/nanostructure gradient interfaces. It suggests ways to mimic these biological features to realize bio-inspired functional surfaces with unique wettability. The book will help researchers innovate designs with novel materials for future scientific works.

Chinese companies are globalizing at an unprecedented rate. Haier is the world's largest telecoms equipment manufacturer; Lenovo is the number one PC maker; and others such as Dalian Wanda, Fosun, Shuanghui and Tencent have been regular players in the global M&A scene. This book presents a detailed analysis of current and emerging Chinese multinationals, and the policies, strategies, and ambitions that are driving them today. Written by leading experts in the field, the book provides the most up-to-date insight into a phenomenon that is rapidly changing the global economic and business landscape.