Advances in Virus Research, Volume 103, the latest in the Advances in Virus Research series, contains new, informative updates on the topic. First published in 1953, this series covers a diverse range of in-depth reviews, providing a valuable overview of the current field of virology. Updates to this release include a Review on phage genes that affect bacterial phenotypes, Phage lysis: new perspectives on the bacterial cell envelope, CRISPRs as the adaptive immune system of archaea and bacteria, CRESS viruses, Tupanviruses, and Giant virus evolution, amongst other topics.

This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field.

This fully revised third edition includes up-to-date topics and developments in the field, which has made tremendous strides since the publication of the second edition in 2004. Many novel techniques based on Next Generation Sequencing have sped up the analysis of fungi and major advances have been made in genome editing, leading to a deeper understanding of the genetics underlying cellular processes as well as their applicability. At the same time, the relevance of fungi is unbroken, both due to the serious threats to human health and welfare posed by fungal pests and pathogens, and to the many benefits that fungal biotechnology can offer for diverse emerging markets and processes that form the basis of the modern bioeconomy. With regard to these advances, the first section of this volume, Genetics, illustrates the basic genetic processes underlying inheritance, cell biology, metabolism and "lifestyles" of fungi. The second section, Biotechnology, addresses the applied side of fungal genetics, ranging from new tools for synthetic biology to the biotechnological potential of fungi from diverse environments. Gathering chapters written by reputed scientists, the book represents an invaluable reference guide for fungal biologists, geneticists and biotechnologists alike.

Introduction to Adsorption: Basics, Analysis, and Applications presents adsorption basics that are relevant and essential to its application, including data analysis, interpretation and design calculations. The book deliberately keeps background information to a minimum, instead comprehensively covering adsorption of liquid solutions, the difference between equilibrium individual solute uptake and surface excess, a general discussion of adsorbate uptake mechanisms and uptake rate expression, uptake steps, performance models and their generalizations, application of performance models, and design methods based on the constant behavior assumption and unused bed length concept.

In the current era current era of significant innovations, science and technology are powerful tools improving human welfare through prosperity and sustainable development. The development of microbiology based industries in any given country is shaped by the characteristics of its technology-particularly its close relation to scientific knowledge, and by country-specific factors such as the level and nature of the scientific knowledge base, the institutional set-up, and the role assumed by the government, all of which influence the country's ability to exploit the new opportunities. This unique book presents an integrated approach for sustained innovation in various areas of microbiology. Focusing on the industrial and socio-legal implications of IPR in microbiological advances, it offers a comprehensive overview not only of the implications of IPR in omics-based research but also of the ethical and intellectual standards and how these can be developed for sustained innovation. The book is divided into three sections discussing current advances in microbiological innovations, recent intellectual property issues in agricultural, and pharmaceutical microbiology respectively. Integrating science and business, it offers a glimpse behind the scenes of the microbiology industry, and provides a detailed analysis of the foundations of the present day industry for students and professionals alike.

This volume provides up-to-date information on the purification and characterization of the enzymes involved in phospholipid biosynthesis and on the mechanisms utilized by cells for the transfer of phospholipids. It also includes protocols for generating phospholipid biosynthetic mutants.

Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states. Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.

This book provides an overview of bidirectional communication between gut-microbiome-brain, pathways, nutrients, and metabolites that are involved in microbiota gut-brain axis (MGBA) interactions. Further it reviews the relevance of this axis in the neurological disorders and potential therapeutic interventions, involving gut microbiome or probiotics and prebiotics which can ameliorate the neurological disorders. The book examines the role of gut microbiota in the establishment and hemostasis of innate immune response and explores the possibility of development of microbiome-targeted therapeutic interventions. Notably, the book discusses the role of the gut microbiota and immune system on the maintenance of brain functions and the development of neurological disorders. It also highlights the recent advances in improving neurological diseases by phytochemicals, prebiotics and probiotics. This book is useful for researchers working in neuropharmacology, Clinical Research, toxicology, neurodegeneration, and stroke biology.

This updated text collects all the introductory aspects of beer brewing science into one place for undergraduate brewing science courses. This expansive and detailed work is written in conversational style, walking students through all the brewing basics from the origin and history of beer to the brewing process to post-brew packaging and quality control and assurance. As an introductory text, this book assumes the reader has no prior knowledge of brewing science and only limited experience with chemistry, biology and physics. The text provides students with all the necessary details of brewing science using a multidisciplinary approach, with a thorough and well-defined program of in-chapter and end-of-chapter problems. As students solve these problems, they will learn how scientists think about beer and brewing and develop a critical thinking approach to addressing concerns in brewing science. As a truly comprehensive introduction to brewing science, Brewing Science: A Multidisciplinary Approach, Second Edition walks students through the entire spectrum of the brewing process. The different styles of beer, the molecular makeup and physical parameters, and how those are modified to provide different flavors are listed. All aspects of the brewery process, from the different setup styles to sterility to the presentation of the final product, are outlined in full. All the important brewing steps and techniques are covered in meticulous detail, including malting, mashing, boiling, fermenting and conditioning. Bringing the brewing process full circle, this text covers packaging aspects for the final product as well, focusing on everything from packaging technology to quality control. Students are also pointed to the future, with coverage of emerging flavor profiles, styles and brewing methods. Each chapter in this textbook includes a sample of related laboratory exercises designed to develop a student's capability to critically think about brewing science. These exercises assume that the student has limited or no previous experience in the laboratory. The tasks outlined explore key topics in each chapter based on typical analyses that may be performed in the brewery. Such exposure to the laboratory portion of a course of study will significantly aid those students interested in a career in brewing science.

R. Fuller 1.1 DEVELOPMENT OF COMMERCIAL PREPARATIONS The history of the probiotic effect has been well documented many times previously (see e.g. Bibel, 1982; Fuller, 1992). The consumption of fermented milks dates from pre-biblical times but the probiotic concept was born at the end of the last century with the work of Metchnikoff at the Pasteur Institute in Paris. In the century that has elapsed since Metchnikoff's work, the probiotic concept has been accepted by scientists and consumers throughout the world. Attempts to refine the practice from the use of traditional soured milks to preparations containing specific micro organisms have occupied the thoughts and endeavours of scientists in many different countries. But, in spite of the large amount of effort expended in attempting to explain and define the effect, it has to be admitted that little is known of the way in which probiotics operate. There are likely to be several different mechanisms because it seems highly improbable that a mode of action that explains resistance to microbial infection will also hold true for improved milk production or alleviation of lactose malabsorption."

Red Wine Technology is a solutions-based approach on the challenges associated with red wine production. It focuses on the technology and biotechnology of red wines, and is ideal for anyone who needs a quick reference on novel ways to increase and improve overall red wine production and innovation. The book provides emerging trends in modern enology, including molecular tools for wine quality and analysis. It includes sections on new ways of maceration extraction, alternative microorganisms for alcoholic fermentation, and malolactic fermentation. Recent studies and technological advancements to improve grape maturity and production are also presented, along with tactics to control PH level. This book is an essential resource for wine producers, researchers, practitioners, technologists and students.

This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo. Despite their initial association with neurodegenerative diseases, there is now increasing evidence of alternative roles for amyloids, with beneficial aspects for cells. In particular, prokaryotes use amyloids as functional assemblies, where they are key players in the cell's physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Bacterial Amyloids: Methods and Protocols is an ideal guide for experts and novices alike to further explore these crucial protein assemblies.

Endophytic Association: What, Why, and How focuses on the endophytic association of plants, how they have originated inside the host, their importance, and how they are beneficial for the environment, as well as humans. The book discusses how using endophytic microbes in agricultural fields can be enriched without impacting environment negatively, and how they can be utilized for pharmaceutical purposes, including bioremediation. It includes advanced and up-to-date information, as well as future directions for young researchers and scientists who are working in the field of agriculture, pharmaceuticals, bio nanotechnology and bioremediation of environmental contaminants for environmental protection and sustainable development.

This book focuses on waterborne pathogens and significant diseases occurring along major rivers around the globe, including key examples like the Amazonas, Mekong River and Nile. Written by leading international experts, it offers unique insights into local riverine infection risks in times of global warming, and addressing these through advances in diagnosis, health management and the development of simple but effective control measures. It also sheds light on why former societies collapsed due to transmitted diseases during periods of climate change, droughts and floods, to help establish effective preventive measures for the future. The book appeals to a wide readership, from scientists in the field of parasitology, infectious diseases and epidemiology, to healthcare managers and general readers with an interest in pathogen spread along the largest rivers on earth. It particularly highlights past and current control mechanisms in times of global warming and assesses potential future health hazards.

Mycorrhiza - the symbiosis between plants and fungi - plays a key role in plant life. This book reviews for the first time the current knowledge of 15 individual genera of ectomycorrhizal fungi. It is unique in that each chapter is dedicated to a single fungal genus, each written by internationally recognized experts on the respective fungal genera. It is thus an invaluable reference source for researchers, students and practitioners in the fields of mycorrhizal biology, mycology, forestry, plant sciences and soil biology.

The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology presents the state-of the-art for MALDI-TOF mass spectrometry. It is a key reference defining how MALDI-TOF mass spectrometry is used in clinical settings as a diagnostic tool of microbial identification and characterization that is based on the detection of a mass of molecules. The book provides updated applications of MALDI-TOF techniques in clinical microbiology, presenting the latest information available on a technology that is now used for rapid microbial identification at relatively low cost, thus offering an alternative to conventional laboratory diagnosis and proteomic identification systems. Although the main use of the technology has, until now, been identification or typing of bacteria from a positive culture, applications in the field of virology, mycology, microbacteriology and resistances are opening up new opportunities.

A new generation of technological vaccines protect against many infectious diseases. This book describes synthetic peptide-based vaccine prototypes - the future of vaccination. Production of peptides becomes simple using automatic synthesizers. Peptides are weak immunogen and need adjuvants to provide an effective autoimmune response, which is why peptide antigens are conjugated with biopolymers and loaded with nanoparticles. The book illustrates the use of peptides vaccine systems and makes predictions of future development not only for infectious diseases, but also for cancers and brain diseases such as Alzheimer, Parkinson and psychiatric diseases. Key Features Summarizes current studies on technological vaccines Describes the uses of vaccines for the prevention of brain diseases Reviews the ways different polymers are used to enhance vaccine efficacy

Integrated Analytical Approaches for Pesticide Management provides proven laboratory practices/examples and methods necessary to control pesticides in food and water in various environments. The book presents insights into good laboratory practices and examples of methods used in individual specialist laboratories, thus enabling stakeholders in the agri-food industry to appreciate the importance of proven, reliable data and the associated quality assurance approaches for end product testing for toxic levels of contaminant residues in food. The book is written in a rigorous, but simple, way to make sure that a broad range of readers can appreciate its technical content. The book's practical nature and generic guidelines distinguish it from others in the marketplace.

This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.

The fundamental concept of The Biology and Identification of the Coccidia (Apicomplexa) of Carnivores of the World is to provide an up-to-date reference guide to the identification, taxonomy, and known biology of apicomplexan intestinal and tissue parasites of carnivores including, but not limited to, geographic distribution, prevalence, sporulation, prepatent and patent periods, site(s) of infection in the definitive and (if known) intermediate hosts, endogenous development, cross-transmission, pathology, phylogeny, and (if known) their treatments. These data will allow easy parasite recognition with a summation of virtually everything now known about the biology of each parasite species covered. The last (very modest) and only treatise published on this subject was in 1981 so this book fills a fundamental gap in our knowledge of what is now known, and what is not, about the coccidian parasites that infect and sometimes kill carnivores and/or their prey that can harbor intermediate stages, including many domestic and game animals.

Great Salt Lake is an enormous terminal lake in the western United States. It is a highly productive ecosystem, which has global significance for millions of migrating birds who rely on this critical feeding station on their journey through the American west. For the human population in the adjacent metropolitan area, this body of water provides a significant economic resource as industries, such as brine shrimp harvesting and mineral extraction, generate jobs and income for the state of Utah. In addition, the lake provides the local population with ecosystem services, especially the creation of mountain snowpack that generates water supply, and the prevention of dust that may impair air quality. As a result of climate change and water diversions for consumptive uses, terminal lakes are shrinking worldwide, and this edited volume is written in this urgent context. This is the first book ever centered on Great Salt Lake biology. Current and novel data presented here paint a comprehensive picture, building on our past understanding and adding complexity. Together, the authors explore this saline lake from the microbial diversity to the invertebrates and the birds who eat them, along a dynamic salinity gradient with unique geochemistry. Some unusual perspectives are included, including the impact of tar seeps on the lake biology and why Great Salt Lake may help us search for life on Mars. Also, we consider the role of human perceptions and our effect on the biology of the lake. The editors made an effort to involve a diversity of experts on the Great Salt Lake system, but also to include unheard voices such as scientists at state agencies or non-profit advocacy organizations. This book is a timely discussion of a terminal lake that is significant, unique, and threatened.

An indispensable primer and reference textbook, the third edition of Geochemical and Biogeochemical Reaction Modeling carries the reader from the field's origins and theoretical underpinnings through to a collection of fully worked examples. A clear exposition of the underlying equations and calculation techniques is balanced by real-world example calculations. The book depicts geochemical reaction modeling as a vibrant field of study applicable to a wide spectrum of issues of scientific, practical, and societal concern. The new edition offers a thorough description of surface complexation modeling, including two- and three-layer methods; broader treatment of kinetic rate laws; the effect of stagnant zones on transport; and techniques for determining gas partial pressures. This handbook demystifies and makes broadly accessible an elegant technique for portraying chemical processes in the geosphere. It will again prove to be invaluable for geochemists, environmental scientists and engineers, aqueous and surface chemists, microbiologists, university teachers, and government regulators.