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Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment > Microscopy
The publication date of the first edition is not stated, but the new edition is apparently considerably revised and expanded. It was written to serve as a multi-purpose text at the senior or graduate level and as a reference for the practicing scientist or engineer. Readers should have a math backgr
Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades. The objective of Optical Nanoscopy and Novel Microscopy Techniques is to choose some of those revolutionary ideas and serve a general audience from broad disciplines to achieve a fundamental understanding of these technologies and to better apply them in their daily research. The book begins with coverage of super-resolution optical microscopy, which discusses targeted modulation such as STED and SIM or localization methods such as PALM. It then discusses novel development of fluorescent probes, such as organic small-molecule probes, fluorescent proteins, and inorganic labels such as quantum dots. Finally, it describes advanced optical microscopy, such as fluorescence lifetime imaging, fiber optic microscopy, scanning ion conductance microscopy, and the joining of optics and acoustics-photoacoustic microscopy. Following each chapter, a detailed list of references is provided. Problems at the end of each chapter are also included.
Recent developments in atomic force microscopy (AFM) have been accomplished through various technical and instrumental innovations, including high-resolution and recognition imaging technology under physiological conditions, fast-scanning AFM, and general methods for cantilever modification and force measurement. All these techniques are now highly powerful not only in material sciences but also in basic biological sciences. There are many nanotechnology books that focus on materials, instruments, and applications in engineering and medicine, but only a few of them are directed toward basic biological sciences. This book tries to bridge this gap. Edited by a prominent researcher, this volume provides an overview of modern AFM technologies: the basic AFM protocols in Part I, newly developed technologies in Part II, and the most recent applications of AFM technologies in biological sciences in Parts III and IV. The chapters are contributed by some of the leading scientists in the field of nanobiology.
The European Federation of Corrosion s Working Party on Surface
Science and the Mechanisms of Corrosion and Protection (EFC WP6)
has defined, as one of its objectives, the development of a
reference material and reference guidelines for the application of
electrochemical scanning tunnelling microscopy (EC-STM) in
corrosion science. The use of EC-STM to study the relationship
between surface structure and surface reactivity in situ on
electrodes in contact with an electrolyte is of major importance in
corrosion research. This report describes the reference material
and procedural guidelines required to use this technique
effectively. STM-users are instructed how to obtain high resolution
data on a carefully prepared copper single-crystal surface.
Stereology is the science that relates three-dimensional structure to the two-dimensional images that can be measured. The most common field of application is in microscopy, both of man-made materials (metals, ceramics, composites, etc.) and of biological tissue samples. This book covers the applications and terminology of both fields. Recent emphasis in stereology is concerned with sampling strategies to avoid bias due to directionality and non-uniformity, and these methods are fully covered. So are the classic techniques that measure size distributions, surface curvature, etc., which are widely useful but not discussed in other recent texts. Finally, most stereology is taught as manual procedures using counting and grids, but this text also covers the use of modern desktop computers for image analysis and processing to obtain and interpret the stereological data.
Provides an account of some of the key developments in the field since the 1992 publication of the monograph Acoustic Microscopy by Oxford U. Press. Among the topics in eight contributed chapters are acoustic microscopy analysis of microelectronic interconnection and packaging technologies; measurin
Two major questions motivate this study: How do new devices get taken up as experimental systems by scientists? How does the adoption of new instruments affect scientific knowledge? Many ramifications emerge from these two simple questions. Among these are historical questions about how, by whom, and why new instruments are introduced, or about how another, different set of instruments might be adopted given alternative social and cultural circumstances. Philosophical questions include the ways in which scientific understanding of the world depends on scientists instruments and techniques. Sociological questions concern such issues as how the organization of work within disciplines and laboratories and other scientific institutions may depend on the equipment employed. All these questions are addressed in this book, which draws upon a range of archival sources as well as published scientific literature, through a detailed historical treatment of the electron microscope s introduction and early impact on the life sciences. The author first describes the introduction of the electron microscope during the World War II years, and then traces its influence on the subsequent divergence of several life sciences research traditions, including what came to constitute cell biology. The historical evidence is discussed in the light of recent discussions on the origin and nature of molecular biology, the importance of new instruments in the postwar life sciences, and the nature of research traditions, among other issues. |
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