This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practicing electron microscopists. He describes currently hot topics such as computer control of microscopes, energy-filtered imaging, cryomicroscopy and environmental microscopy, digital imaging, high resolution scanning, and transmission microscopy. The author has expanded the highly praised case studies of the first edition to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years' practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology.

The investigation and manipulation of matter on the atomic scale have been revolutionized by scanning tunneling microscopy and related scanning probe techniques. This book is the first to provide a clear and comprehensive introduction to this subject. Beginning with the theoretical background of scanning tunneling microscopy, the design and instrumentation of practical STM and associated systems are described in detail, including topographic imaging, local tunneling barrier height measurements, tunneling spectroscopy, and local potentiometry. A treatment of the experimental techniques used in scanning force microscopy and other scanning probe techniques rounds out this section. The second part discusses representative applications of these techniques in fields such as condensed matter physics, chemistry, materials science, biology, and nanotechnology, so this book will be extremely valuable to upper-division students and researchers in these areas.

This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline 'tricks of the trade'. The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semi-thin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immuno electron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples. This manual will be an invaluable guide to anyone using electron microscopy on human and animal tissue and wishing to develop a routine that guarantees good and reproducible results.

The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.

This book traces the historical development of microscopy instruments from their invention to the current state of the art. New concepts and engineering solutions are presented for modern light microscopes, with a focus on the practical construction of optical systems. Real design parameters of dioptric objectives and other systems are provided to supply readers with basic information for independent designs. Full-color photomicrographs of real objects illustrate the quality of aberration correction that is required from optics.