Over the past decade, neuroproteomics has shed light on the molecular features of schizophrenia by depicting biological processes involved with its establishment, maintenance and treatment. These studies have also pointed to potential biomarkers applicable to diagnosis and medication monitoring. Edited by a leader in the field of neuroproteomics with contributions from subject experts, this new volume will address recent findings and compile evidence from difference perspectives-such as human samples, animal models, pluripotent stem cell-derived in vitro pre-clinical models-and provide findings to inform the development of innovative future treatment strategies. This volume will be useful for a broad audience of researchers and professionals, including biologists, neurologists, psychiatrists, analytical chemists, and pharmacists, among others.

This book provides an interdisciplinary look at emerging trends in signal processing and biomedicine found at the intersection of healthcare, engineering, and computer science. Bringing together expanded versions of selected papers presented at the 2020 IEEE Signal Processing in Medicine and Biology Symposium (IEEE SPMB), it examines the vital role signal processing plays in enabling a new generation of technology based on big data and looks at applications ranging from medical electronics to data mining of electronic medical records. Topics covered include analysis of medical images, machine learning, biomedical nanosensors, wireless technologies, and instrumentation and electrical stimulation. Biomedical Sensing and Analysis: Signal Processing in Medicine and Biology presents tutorials and examples of successful applications, and will appeal to a wide range of professionals, researchers, and students interested in applications of signal processing, medicine, and biology. Presents an interdisciplinary look at research trends in signal processing and biomedicine; Promotes collaboration between healthcare practitioners and signal processing researchers; Includes tutorials and examples of successful applications.

This book is the first comprehensive compilation describing the importance of sandalwood in national and international markets, genetic resources, molecular markers, whole genome sequencing, and pathway genes involved in oil biosynthesis, aroma and fragrance. Application of various "omics" approaches in delineating genome architecture and annotation of genes is highlighted. This book comprises 10 chapters covered over 200 pages authored by the researchers involved in sandalwood genomics. The sandalwood, Santalum album is known for its unique fragrance and finest wood available for carving. Also, sandalwood is intertwined with Indian culture and it is the second most valuable and expensive tree in the world.

Written by a group of world-renowned virologists, this book reviews all of the most recent advances in rhabdovirology, providing an overiew of the field.

Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra. Ancient DNA research really gained momentum following the invention of PCR, which allowed millions of copies to be made of the few remaining DNA molecules preserved in fossils and museum specimens. In Ancient DNA: Methods and Protocols expert researchers in the field describe many of the protocols that are now commonly used to study ancient DNA. These include instructions for setting up an ancient DNA laboratory, extraction protocols for a wide range of different substrates, details of laboratory techniques including PCR and NGS library preparation, and suggestions for appropriate analytical approaches to make sense of the sequences obtained. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ancient DNA: Methods and Protocols seeks to aid scientists in the further study of ancient DNA and the methodological approaches in ancient research.

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Following the two meetings on Lactoferrin Structure and Function that were held in Honolulu, Hawaii, in 1993 and 1995, the Third International Conference on Lactoferrin Structure and Function was held in Le Touquet, France, and has successfully reinforced and diversified the previously created bridges between biochemists, clinicians, and companies. In fact, scientists, physicians, and people of industry from different domains have brought a wealth of recent information concerning biochemistry and technical advances in the identification of lactoferrin-derived compounds as well as cell biology, molecular biol ogy, pathology, and medical applications of lactoferrin and lactoferrin-derived com pounds. We were so delighted with the rapid growth of knowledge concerning many biologi cal and immunological functions of lactoferrins and the relationships between their struc ture and function, we wanted to share our pleasure with the readers interested in this field. The present book. which represents a review of some of the most exciting contributions, is intended to reflect the status of our knowledge and transmit our hopes for the future devel opment of in vivo applications of natural and recombinant lactoferrins. We would like to express our gratitude to the sponsors who contributed to the or ganization of the meeting in such a pleasant place and allowed the participation of several young researchers. We would also like to thank all the participants who have answered with enthusiasm our invitation and to every one of the Laboratoire de Chimie Biologique for the constant and efficient help."

For volume 1 alone:
A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
For both volumes:
A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer.
Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-findmethods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

Drawing on contributions from more than thirty scholars and experts in the field, this book examines the role of business as an enabler, as an inhibitor, and ultimately as a co-actor in global sustainability transformations expected over the next few decades. The Role of Business in Global Sustainability Transformations employs several theoretical perspectives and provides abundant examples and cases to discuss a variety of emerging concepts, phenomena, and trends shaping business sustainability. Weaving through the chapters, the editors present core tensions and sources of inertia towards transformative change, and acknowledge that envisioning multiple solutions and pathways are possible and desirable. They advocate for the need to align visions, actions and time horizons between policy, society and business in addressing the interlinked socio-ecological challenges that our society currently faces. This book will be an important resource for scholars and professionals working in the field of sustainability and sustainable business, and a vital educational text for students interested in this discipline.

The 1st World Congress on Geriatrics and Neurodegenerative Disease Research (GeNeDis 2014), will focus on recent advances in geriatrics and neurodegeneration, ranging from basic science to clinical and pharmaceutical developments and will provide an international forum for the latest scientific discoveries, medical practices and care initiatives. Advanced information technologies will be discussed concerning the various research, implementation and policy, as well as European and global issues in the funding of long-term care and medico-social policies regarding elderly people. GeNeDis 2014 takes place in Corfu Greece, 10-13 April 2014. This volume focuses on the sessions from the conference on computational biology and bioinformatics.

Bacteriophages (viruses that infect bacteria) are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular biology. Phage can confer key phenotypes on their host for example, converting a non-pathogenic strain into a pathogen and they play a key role in regulating bacterial populations in all sorts of environments. The phage-bacterium relationship varies enormously, from the simple predator-prey model to a complex, almost symbiotic relationship that promotes the survival and evolutionary success of both. While infection of bacteria used in the fermentation industry can be very problematic and result in financial losses, in other scenarios, phage infection of bacteria can be exploited for industrial and/or medical applications. Interest in phage and phage gene products as potential therapeutic agents is increasing rapidly and is likely to have a profound impact on the pharmaceutical industry and biotechnology in general over the comi

Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more.

This text attempts to introduce the molecular biology of cell membranes to students and professionals of diverse backgrounds. Although several membrane biology books are available, they do not integrate recent knowledge gained using modern molecular tools with more traditional membrane topics. Molecular techniques, such as cDNA cloning and x-ray diffraction, have provided fresh insights into cell membrane structure and function. The great excitement today, which I attempt to convey in this book, is that molecular details are beginning to merge with physiological responses. In other words, we are beginning to understand precisely how membranes work. This textbook is appropriate for upper-level undergraduate or beginning graduate students. Readers should have previous or concurrent coursework in biochemistry; prior studies in elementary physiology would be helpful. I have found that the presentation of topics in this book is appropriate for students of biology, biochemistry, biophysics and physiology, chemistry, and medicine. This book will be useful in courses focusing on membranes and as a supplementary text in biochemistry courses. Professionals will also find this to be a useful resource book for their personal libraries.

This book focuses on the multi-omics big-data integration, the data-mining techniques and the cutting-edge omics researches in principles and applications for a deep understanding of Traditional Chinese Medicine (TCM) and diseases from the following aspects: (1) Basics about multi-omics data and analytical methods for TCM and diseases. (2) The needs of omics studies in TCM researches, and the basic background of omics research in TCM and disease. (3) Better understanding of the multi-omics big-data integration techniques. (4) Better understanding of the multi-omics big-data mining techniques, as well as with different applications, for most insights from these omics data for TCM and disease researches. (5) TCM preparation quality control for checking both prescribed and unexpected ingredients including biological and chemical ingredients. (6) TCM preparation source tracking. (7) TCM preparation network pharmacology analysis. (8) TCM analysis data resources, web services, and visualizations. (9) TCM geoherbalism examination and authentic TCM identification. Traditional Chinese Medicine has been in existence for several thousands of years, and only in recent tens of years have we realized that the researches on TCM could be profoundly boosted by the omics technologies. Devised as a book on TCM and disease researches in the omics age, this book has put the focus on data integration and data mining methods for multi-omics researches, which will be explained in detail and with supportive examples the "What", "Why" and "How" of omics on TCM related researches. It is an attempt to bridge the gap between TCM related multi-omics big data, and the data-mining techniques, for best practice of contemporary bioinformatics and in-depth insights on the TCM related questions.

"COVID-19 and Omics Technologies" is a comprehensive, integrative assessment of recent information and knowledge collected on SARS-CoV-2 and COVID-19 during the pandemic based on omics technologies. It demonstrates how omics technologies could better investigate the infectious disease and propose solutions to the current concerns. The value of multi-omics technologies in understanding disease etiology and host response, discovering infection biomarkers and illness prediction, identifying vaccine candidates, discovering therapeutic targets, and tracing pathogen evolution is discussed in this book. These factors combine to make it a valuable resource to enhance understanding of both "Omics technology" and "COVID-19" as a disease. The book covers the most recent understanding of COVID-19 and the applications of cutting-edge studies, making it accessible to a large multidisciplinary readership. The book explains how high-throughput technologies and systems biology might assist to solve the pandemic's challenges and deconstruct and appreciate the substantial contributions that omics technologies have made in predicting the path of this unforeseeable pandemic. Features: In-depth summary of clinical presentation, epidemiological impact, and long-term sequelae of COVID-19 pandemic. A systematic overview of omics-based approaches to the study of COVID-19 biology. Recent research results and some pointers to future advancements in methodologies used. Detailed examples from recent studies on COVID-19 encompassing different omics methodologies. A detailed description of methodologies and notes on the applications of state-of-the-art technologies. This book is intended for scientists who need to understand the biology of COVID-19 from the perspective of omics investigations, as well as researchers who want to employ omics-based technologies in disease biology.

Sustainable Plant Nutrition: Molecular Interventions and Advancements for Crop Improvement explores the significant opportunities for sustainable, eco-friendly approaches in plant nutrition and agricultural crop production. The book highlights the various prospects involved in optimizing plant nutrient uptake agriculture and includes chapters representing diverse areas dealing with biotechnology, nanotechnology, molecular biology, proteomics, genomics and metabolomics. This book is an ideal resource for those seeking to ensure a sustainable plant production future. While plants have evolved a set of elaborate mechanisms to cope with nutrient limitations, the traditional supplementation by the application of fertilizers to plant productivity may then lead to overfertilization which can actually reduce plant growth and have adverse effects on the environment. To tackle these issues, a detailed understanding of the responses of plants to nutrients and nutrient deficiency at the physiological, metabolic, transcriptome and epigenetic level is essential.

Documents the confused early history of DNA, RNA and proteins - a transformative history of molecular biology like no other. Integrates the influences of biochemistry and genetics on the landscape of molecular biology. Chronicles the important discoveries, preconceptions and misconceptions that retarded or misdirected progress. Highlights major pioneers and contributors to molecular biology, with a focus on RNA and noncoding DNA. Summarizes the mounting evidence for the central roles of non-protein-coding RNA in cell and developmental biology. Provides a thought-provoking retrospective and forward-looking perspective for advanced students and professional researchers.

Presents potential biotechnological topics and strategies for the valuation of agricultural waste materials Provides technical concepts on the production of various commercially significant bio-products Introduces various microbial bioprocesses to sustainably valorise various potential wastes as renewable feedstocks for production of biofuels and biochemicals Explores the relevant scale-up opportunities, commercialization aspects and critical technological advances Explains concepts and recent trends in life cycle analyses in waste valorization

Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluor- cent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a pro- nent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Protocols for the detection of chromosomal re- rangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis.

Mycotoxins produced by molds are common contaminants of many important crops, including wheat, corn, rice, and peanuts. Some mycotoxins are found in fruits and vegetables. These contaminants have a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. The occurrence of mycotoxins in foods is an unavoidable worldwide problem. About 80 countries have imposed regulatory limits to minimize human and animal exposure to mycotoxins. Regulatory limits, including international standards, have tremendous economic impact and must be developed using science-based risk assessments. The purpose of Mycotoxin Protocols is to provide the scientific and technological basis for analytical methods for use in obtaining the exposure data needed for risk assessments. Mycotoxin Protocols is divided into four sections, which are interc- nected. The first section: Chapters 1-5 describe the general techniques for mycotoxin analysis with emphasis on the importance of method validation based on statistical parameters; sampling procedures for collecting a sample as representative as possible of a bulk lot; the isolation of mycotoxins for use as analytical standards or for toxicological studies; the evaluation of purity and preparation of standards; and the detection and identification of impu- ties in isolated mycotoxins. Sections 2-4: Chapters 6-19 describe the most current chromatographic and immunochemical methods for studies on the major mycotoxins.

This is a book about software engineering, bioinformatics, the C++ programming language and the SeqAn library. In the broadest sense, it will help the reader create better, faster and more reliable software by deepening their understanding of available tools, language features, techniques and design patterns. Every developer who previously worked with C++ will enjoy the in-depth chapter on important changes in the language from C++11 up to and including C++20. In contrast to many resources on Modern C++ that present new features only in small isolated examples, this book represents a more holistic approach: readers will understand the relevance of new features and how they interact in the context of a large software project and not just within a "toy example". Previous experience in creating software with C++ is highly recommended to fully appreciate these aspects. SeqAn3 is a new, re-designed software library. The conception and implementation process is detailed in this book, including a critical reflection on the previous versions of the library. This is particularly helpful to readers who are about to create a large software project themselves, or who are planning a major overhaul of an existing library or framework. While the focus of the book is clearly on software development and design, it also touches on various organisational and administrative aspects like licensing, dependency management and quality control.

DNA Damage and Double Strand Breaks, Volume 51 in The Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters that provide an update on female and male genital schistosomiasis and a call to integrate efforts to escalate diagnosis, treatment and awareness in endemic and non-endemic settings, vertebrates as uninfected disseminators of helminth eggs and larvae, and combatting anthelmintic resistance in ruminants.