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Books > Science & Mathematics > Biology, life sciences > Biochemistry
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Protein NMR Techniques (Paperback, Softcover reprint of hardcover 2nd ed. 2004)
Loot Price: R4,471
Discovery Miles 44 710
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Protein NMR Techniques (Paperback, Softcover reprint of hardcover 2nd ed. 2004)
Series: Methods in Molecular Biology, 278
Expected to ship within 10 - 15 working days
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When I was asked to edit the second edition of Protein NMR
Techniques, my first thought was that the time was ripe for a new
edition. The past several years have seen a surge in the
development of novel methods that are truly revolutionizing our
ability to characterize biological macromolecules in terms of
speed, accuracy, and size limitations. I was particularly excited
at the prospect of making these techniques accessible to all NMR
labs and for the opportunity to ask the experts to divulge their
hints and tips and to write, practically, about the methods. I
commissioned 19 chapters with wide scope for Protein NMR
Techniques, and the volume has been organized with numerous themes
in mind. Chapters 1 and 2 deal with recombinant protein expression
using two organisms, E. coli and P. pastoris, that can produce high
yields of isotopically labeled protein at a reasonable cost.
Staying with the idea of isotopic labeling, Chapter 3 describes
methods for perdeuteration and site-specific protonation and is the
first of several chapters in the book that is relevant to studies
of higher molecular weight systems. A different, but equally
powerful, method that uses molecular biology to "edit" the spectrum
of a large molecule using segmental labeling is presented in
Chapter 4. Having successfully produced a high molecular weight
target for study, the next logical step is data acquisition. Hence,
the final chapter on this theme, Chapter 5, describes TROSY methods
for stru- ural studies.
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