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Lipids and Related Compounds (Paperback, Softcover reprint of the original 1st ed. 1988)
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Lipids and Related Compounds (Paperback, Softcover reprint of the original 1st ed. 1988)
Series: Neuromethods, 7
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Lipids m the nervous system are major components of the m- branes.
The presence of glycolipids in high concentrations is unique for
the nervous system. Recent discoverres of the functronal and
pathological importance of lipids such as diacylglycerols,
polyphosphoinositides, prostaglandins, leukotrienes,
docosahexaenoic acid, platelet activating factor, and gangliosides
have markedly increased the number of publications on nervous
system lipids. Many new methods have been developed. Thus, there IS
a need for this volume that is dedicated to that methodo- gy. This
volume places all of the methods for lipids into perspective with
recommendations concerning the selection of a method for a specific
purpose. Lloyd A. Horrocks vii Contents . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . Preface to the Series vi Preface.. . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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..*............................... xix List of Contributors Lipid
Extraction Norman S. Radin 1 1. Introduction
......................................................
......................................... 5 2. Properties of
Solvents 2.1. Ethers
........................................................ 6 8 2.2.
Alcohols ..................................................... 9
2.3. Halocarbons ................................................ 9
2.4. Hydrocarbons .............................................
2.5. Miscellaneous Solvents ................................ 10
.................................... 10 3. Storage of Lipid
Extracts 14 4. Homogenizers
.................................................. ..... 15 5.
Separating the Extract from the Tissue Residue 15 5.1. Use of
Filtration .......................................... 16 5.2.
Centrifugation ............................................
.............. 17 6. Removing Nonlipids from Lipid Extracts 6.1.
Removal of Nonlipids by Evaporation and Reextraction
............................................... 17
.................... 17 6.2. Prewashing the Tissue Sample.. 6.3.
Removal of Nonlipids by Precipitating the Lipids with the Proteins
............................... 18 ............................ 19
6.4. Liquid/Liquid Partitioning .................. 19 6.5. Removal
of Nonlipids by Dialysis 6.6. Separation from Nonlipids by Changing
a 21 Partition Constant .......................................
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