The second edition of Adhesion Protein Protocols combines
traditional
techniques with cutting-edge and novel techniques that can be
adapted
easily to different molecules and cell types. The topics discussed
in
this updated second edition include novel techniques for
studying
cell-cell adhesion, neutrophil chemotaxis, in vitro assays used
to
study leukocyte migration through monolayers of cultured
endothelial
cells, and novel techniques to purify pseudopodia from migratory
cells.
This book also discusses the study of cell-matrix interaction,
RNA
interference, fluorescence recovery after photobleaching,
actin
purification, the application of microarray techniques, and the
role of
adhesion proteins in the study of proteomics.
The protocols discussed in this volume are suitable for both novice
and
expert scientists, who will gain further insight into the complex
and
incompletely understood processes involved in cellular
adhesion.
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