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Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
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Genetic Engineering (Hardcover)
Loot Price: R2,212
Discovery Miles 22 120
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Genetic Engineering (Hardcover)
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This book has a distinguishing feature of having condensed material
with adequate information on genetic engineering especially of the
microbes. The book covers almost all the topics of genetic
engineering for the graduate, postgraduate students and young
research scholars of biological sciences. The book is written as
per syllabus of genetic engineering paper for Masters course in
biotechnology, biochemistry, life sciences of most of the
universities. The book is much useful for the students of Masters
degree. Emphasis is given on the basic fundamentals. The book
contains twelve chapters starting from ' Isolation, purification
and estimation of nucleic acids' as chapter 1. The chapter
describes general techniques for the isolation and purification of
DNA as well as RNA. It also describes methods for quantitative
estimation of the nucleic acids. The second chapter describes
general characteristics of the vectors used in genetic engineering
and also the general account of commonly used individual vectors.
The chapter also describes expression vectors. The third chapter
describes various commonly used restriction endonucleases. The
fourth chapter describes commonly used enzymes in genetic
engineering viz. Reverse transcriptase, DNA polymerase I,
polynucleotide kinase, teminal dcoxynucleotidyl transferase,
alkaline phosphatase, SI nuclease, DNA ligase etc. The fifth
chapter describes electrophoresis for the separation of nucleic
acids fragments. The sixth chapter is of cloning strategies. It
describes construction of genomic DNA library , chromosomal
walking, cDNA library, cDNA cloning. The seventh chapter describes
DNA sequencing techniques and includes chemical modification method
of Maxam and Gilbert, dideoxy sequencing method of Sanger,
modifications of chain terminator sequencing, analysis of the
sequencing data. The eighth chapter includes various methods of
site directed mutagenesis. The ninth chapter describes polymerase
chain reaction (PCR). It also includes primer designing and various
types of polymerase chain reactions viz. reverse transcriptase
polymerase chain reaction (RT-PCR), nested PCR, multiplex PCR etc.
Besides, there are chapters 10, 11 and 12 on gene therapy, human
genome and proteomics. At the end, glossary has been put which
explains main terms used in genetic engineering. One of the
important factor introduced in the book is the chapter structure
given in the beginning of each chapter that provides, at a glance,
the contents of the whole chapter which offers a better learning
mechanism. Each chapter is also presented with an introduction that
covers the concept of the whole chapter in brief and offers clear
understanding of the subject matter to the students. The author on
the basis of his experience in teaching genetic engineering at the
university level for more than a decade has offered the text in an
easily understandable form to the postgraduate students. The book
should be of invaluable help to the students, researchers and all
those interested in understanding genetic engineering.
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