A panel of outstanding investigators surveys and explains the major
cutting-edge methods used in thryroid receptor (TR) research and
explains their practical experimental details. Described in
step-by-step detail to ensure robust experimental results, the
techniques presented cover a wide variety of key areas, including
TR in development and knockout (mouse and Xenopus), transcriptional
regulation by TRs in both cell-free systems and in living cells,
and TR mutant analysis of patients. Additional methods provide
powerful tools for the isolation of TR-regulated protein complexes,
for studying the oncogene v-Erba in blood cell differentiation, and
for target gene analysis in the brain. Microarray chip methods are
also presented for analyzing the organs of transgenic mice to
identify target genes in the liver.
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